Epidermal growth factor (EGF) is a small mitogenic polypeptide that stimulates the proliferation and differentiation of a wide variety of cells of ectodermal and mesodermal origin, including fibroblasts, epithelial cells, and endothelial cells.
MACS GMP Recombinant Human EGF is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Data and images for
MACS
®
GMP Recombinant Human EGF

Figures

Figure 1

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Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human EGF.
The peak corresponds to the calculated molecular mass of 6347 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human EGF.
The peak corresponds to the calculated molecular mass of 6347 Da.

Figure 2

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MACS GMP Recombinant Human EGF biological activity.
Activity of Human EGF, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 2

MACS GMP Recombinant Human EGF biological activity.
Activity of Human EGF, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 3

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MACS GMP Recombinant Human EGF batch-to-batch variability.
Activity of three different production batches of Human MACS GMP EGF.

Figure 3

MACS GMP Recombinant Human EGF batch-to-batch variability.
Activity of three different production batches of Human MACS GMP EGF.

Specifications for
MACS
®
GMP Recombinant Human EGF

Overview

Epidermal growth factor (EGF) is a small mitogenic polypeptide that stimulates the proliferation and differentiation of a wide variety of cells of ectodermal and mesodermal origin, including fibroblasts, epithelial cells, and endothelial cells.
MACS GMP Recombinant Human EGF is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

EGF is the prototype of the large family of EGF-like proteins with a common structural motif comprising three intramolecular disulfide bonds. EGF is produced by various cell types like mammary gland cells, gut epithelial cells, and cells in the nervous system and the kidney. Production of EGF is induced by testosterone and inhibited by estrogens.
In vitro
, EGF stimulates the proliferation and differentiation of mesenchymal cells, acts as a mitogen for fibroblasts, neural precursors, epithelial and endothelial cells, and promotes colony formation of epidermal cells.

Applications

MACS GMP Recombinant Human EGF can be used as an ingredient for many cell culture applications. EGF can support the maintenance of ectodermal and mesodermal progenitor cells, and stimulates the proliferation and differentiation of a wide variety of cell types deriving both from ectoderm and mesoderm.

Biological activity

  • The specific activity is determined by proliferation assay according to Robinson and Gaines-Das
    1
    using 3T3 cells. The proliferation assay was calibrated with the international standard for human EGF (NIBSC code 91/530) provided by the National Institute for Biological Standards and Control.
  • Specific activity: ≥ 5×
    10
    5
    IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP Recombinant Human EGF

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for
MACS
®
GMP Recombinant Human EGF

Publications

  1. Robinson, C. J. and Gaines-Das, R. (1996)
    The international standard for epidermal growth factor (EGF): comparison of candidate preparations by
    in vitro
    bioassays and immunoassays. National Institute for Biological Standards and Control.
    Growth Factors 13: 163-170

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®
GMP Recombinant Human EGF

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