Background information
Activin A is a member of the TGF-β superfamily and is involved in a wide range of biological processes like growth and differentiation of several tissues. It has been described to affect embryogenesis and hematopoiesis and is necessary for the maintenance of self-renewal and pluripotency of human embryonic stem cells (hESCs).
It supports long-term feeder and serum-free growth of hESCs.
Activin A induces the expression of Oct4, Nanog, Nodal, Wnt3, FGF-2, and FGF-8 and suppresses the BMP signal. It also controls the expression and secretion of hormones like follicle stimulating hormone (FSH), prolactin, and ACTH (corticotropin).
The amino acid sequence of human, mouse, and rat activin A shares 100% identity.
Applications
Human Activin A can be used for a variety of applications, including:
- Maintenance of pluripotency of embryonic stem cells under feeder- and serum-free conditions.
- Differentiation of embryonic stem cells and iPS cells.
- Stimulation and differentiation of mesenchymal cells.
Technical specifications- Identity/ Molecular mass: 25934 Da, determined by mass spectrometry. This corresponds to the mature form of Activin A with two identical, non-glycosylated disulfide-linked polypeptide chains (116 amino acid residues).
- Purity: ≥ 90 % as determined by chip electrophoresis.
- Endotoxin content: < 50 EU/mg, as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay (Pharmacopoeia Europaea (Ph. Eur.)).
- Residual host cell DNA content: < 20 ng/mg, as determined by quantitative PCR specific for CHO genomic DNA.
- Residual host cell protein content: < 25 µg/mg, as determined by CHO HCP ELISA.
The product quality certificate of MACS
® GMP Recombinant Human Activin A was adapted. Learn more
here.Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
