Human Activin A is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
MACS GMP Recombinant Human Activin A is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Data and images for
MACS
®
GMP Recombinant Human Activin A

Figures

Figure 1

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The biological activity of MACS GMP Human Activin A was determined by inhibition assay using MPC-11 cells.

Figure 1

The biological activity of MACS GMP Human Activin A was determined by inhibition assay using MPC-11 cells.

Figure 2

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MACS GMP Recombinant Human Activin A biological activity.
Activity of Human Activin A, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 2

MACS GMP Recombinant Human Activin A biological activity.
Activity of Human Activin A, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Specifications for
MACS
®
GMP Recombinant Human Activin A

Overview

Human Activin A is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
MACS GMP Recombinant Human Activin A is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

Activin A is a member of the TGF-β superfamily and is involved in a wide range of biological processes like growth and differentiation of several tissues. It has been described to affect embryogenesis and hematopoiesis and is necessary for the maintenance of self-renewal and pluripotency of human embryonic stem cells (hESCs).
It supports long-term feeder and serum-free growth of hESCs.
Activin A induces the expression of Oct4, Nanog, Nodal, Wnt3, FGF-2, and FGF-8 and suppresses the BMP signal. It also controls the expression and secretion of hormones like follicle stimulating hormone (FSH), prolactin, and ACTH (corticotropin).
The amino acid sequence of human, mouse, and rat activin A shares 100% identity.

Applications

Human Activin A can be used for a variety of applications, including:
  • Maintenance of pluripotency of embryonic stem cells under feeder- and serum-free conditions.
  • Differentiation of embryonic stem cells and iPS cells.
  • Stimulation and differentiation of mesenchymal cells.

Biological activity

  • The specific activity is determined by inhibition assay using murine MPC-11 cells according to Phillips et al.
    1
    , The inhibition assay was calibrated with the reference reagent for human Activin A (NIBSC code 91/626) provided by the WHO/National Institute for Biological Standards and Control.
  • Specific activity: ≥ 1×
    10
    3
    U/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP Recombinant Human Activin A

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for
MACS
®
GMP Recombinant Human Activin A

Publications

  1. Phillips, D. J. et al. (1999)
    A sensitive and specific
    in vitro
    bioassay for activin using a mouse plasmacytoma cell line, MPC-11.
    J. Endocrinol. 162(1): 111-116

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