Clone:
145.15.3.8C12
Isotype:
mouse IgG1κ
Alternative names:
L-selectin, LAM-1, Leu-8, LECAM1, MEL-14, LEU8, TQ1

Data and images for
MACS
®
GMP CD62L Fluorescent Antibodies

Figures

Figure 1

A leukapheresis sample was labeled with MACS
®
GMP CD62L-PE, CD3-VioGreen™ and MACS
®
GMP CD8-APC for gating purposes. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Viable lymphocytes were further gated on CD3
+
cells.
View details

Figure 1

A leukapheresis sample was labeled with MACS
®
GMP CD62L-PE, CD3-VioGreen™ and MACS
®
GMP CD8-APC for gating purposes. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Viable lymphocytes were further gated on CD3
+
cells.

Specifications for
MACS
®
GMP CD62L Fluorescent Antibodies

Overview

MACS
®
GMP CD62L Fluorescent Antibodies consist of a mouse monoclonal antibody against the human CD62L antigen conjugated to a fluorochrome. They are developed for flow cytometric identification and flow cytometric sorting of CD62L-positive cells.

Alternative names

L-selectin, LAM-1, Leu-8, LECAM1, MEL-14, LEU8, TQ1

Detailed product information

Background information

Clone 145/15 recognizes the human CD62L antigen. Most hematopoietic cells express CD62L, including many peripheral blood B cells, T cells, monocytes, granulocytes, and some myeloid cells from bone marrow, and thymocytes. CD62L is continuously cleaved from the cell surface of neutrophils and lymphocytes by endoproteases. CD62L is important for homing of naive lymphocytes via endothelial venules to peripheral lymph nodes and Peyer’s patches.

Applications

MACS
®
GMP CD62L Fluorescent Antibodies are for identification of CD62L-positive cells by flow cytometry as well as cell enrichment by flow cytometric sorting.

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for
ex vivo
cell processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

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