CD135 (FLT3) antibodies, mouse

CD135 (FLT3) antibodies, mouse

Clone: REA779 | Dilution: 1:50
Clone REA779 recognizes the mouse CD135 antigen, a 150 kDa surface protein Fms-like tyrosine kinase receptor 3 (FLT3), also known as FLK-2. CD135 has functional and structural homology to the class III–receptor tyrosine kinases that include the M-CSF-, KIT-, and PDGF-receptors. The receptor is expressed on progenitor cells in adult bone marrow, thymus, brain, testis, and placenta. All hematopoietic cells develop from CD135 negative hematopoietic stem cells (HSCs) through CD135 positive progenitor cells. In synergy with other growth factors like G-CSF, GM-CSF, SCF, and IL-3, ligation of CD135 with FLT3 ligand promotes the proliferation and differentiation of primitive hematopoietic stem cells. As a result early B cell lineage differentiation as well as expansion of monocytes and immature dendritic cells are stimulated.
Additional information: Clone REA779 displays negligible binding to Fc receptors.

Alternative names

FLT3, FLK-2, Ly72, wmfl, Flt3/Flk2, STK-1

Technical specifications

  • Antigen: CD135 (FLT3)
  • Clone: REA779
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: FLT3, FLK-2, Ly72, wmfl, Flt3/Flk2, STK-1
  • Entrez Gene ID: 14255
  • Molecular mass of antigen [kDa]: 110
  • Distribution of antigen: B cells, bone marrow, brain, CNS cells, hematopoietic cells, lung, spleen
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, PE-Vio770, Biotin
  • Selected references

    1. Hannum, C. et al. (1994) Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs. Nature 368(6472): 643-648
    2. Ogawa, M. et al. (1998) Flt3/Flk-2 and c-Kit are not essential for the proliferation of B lymphoid progenitor cells in the bone marrow of the adult mouse. Exp. Hematol. 26(6): 478-488
    3. Waskow, C. et al. (2008) The receptor tyrosine kinase Flt3 is required for dendritic cell development in peripheral lymphoid tissues. Nat. Immunol. 9(6): 676-683
    4. Auffray, C. et al. (2009)
      CX
      3
      CR1
      +
      CD115
      +
      CD135
      +
      common macrophage/DC precursors and the role of CX
      3
      CR1 in their response to inflammation.
      J. Exp. Med. 206(3): 595-606
    5. Böiers, C. et al. (2010) Expression and role of FLT3 in regulation of the earliest stage of normal granulocyte-monocyte progenitor development. Blood 115(24): 5061-5068
    6. Boyer, S. W. et al. (2011) All hematopoietic cells develop from hematopoietic stem cells through Flk2/Flt3-positive progenitor cells. Cell Stem Cell 9(1): 64-73
  • Customer reports and application notes

Product options: 8
30 µg in 200 µL (1)
EUR  90,00
150 µg in 1 mL (1)
EUR  265,00
30 µg in 200 µL (1)
EUR  90,00
150 µg in 1 mL (1)
EUR  265,00
30 µg in 200 µL (1)
EUR  120,00
150 µg in 1 mL (1)
EUR  345,00
30 µg in 200 µL (1)
EUR  50,00
150 µg in 1 mL (1)
EUR  140,00

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