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Splenocytes from BALB/c mice were either left unstimulated (left image) or stimulated with CD3/CD28 antibodies at 37 °C for 48 hours. Cells were stained with CD134 (OX40) antibodies as well as with CD4 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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