Anti-LAT pY171 antibodies, human

Anti-LAT pY171 antibodies, human

Clone: REA160
Clone REA160 recognizes the human linker for activation of T cell family member 1 (LAT) antigen phosphorylated at tyrosine 171 (pY171). LAT is an integral membrane protein that was initially detected as a major PTK substrate during T cell–receptor (TCR) activation. It is also involved in low affinity immunoglobulin gamma Fc region receptor III mediated signaling in natural killer cells and high affinity immunoglobulin epsilon receptor mediated signaling in mast cells. TCR-mediated LAT phosphorylation is critical for the membrane recruitment of signaling complexes required for T cell–activation. The LAT protein contains a short extracellular domain, a transmembrane sequence, and an extended cytoplasmic domain with nine tyrosine residues conserved in the mouse and human sequences. Some of these tyrosine residues have been shown to be docking sites for mediators of TCR signaling. Tyrosine 171 is suggested to be involved in more than merely creating SH2-binding sites and may affect the native conformation, localization, stability, and/or sequential phosphorylation of LAT during TCR stimulation.
Additional information: Clone REA160 displays negligible binding to Fc receptors.

Alternative names

LAT1, pp36, p36-38

Technical specifications

  • Antigen: LAT pY171
  • Clone: REA160
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (I) antibodies
  • Alternative names of antigen: LAT1, pp36, p36-38
  • Entrez Gene ID: 27040
  • Molecular mass of antigen [kDa]: 28
  • Distribution of antigen: NK cells, T cells, mast cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC
  • Selected references

    1. Zhang, W. et al. (1998) LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation. Cell 92(1): 83-92
    2. Paz, P. E. et al. (2001) Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells. Biochem. J. 356(Pt 2): 461-471
    3. Larghi, P. et al. (2013) VAMP7 controls T cell activation by regulating the recruitment and phosphorylation of vesicular Lat at TCR-activation sites. Nat. Immunol. 14(7): 723-731
Product options: 4
for 30
tests
(1)
EUR  125,00
for 100
tests
(1)
EUR  310,00
for 30
tests
(1)
EUR  125,00
for 100
tests
(1)
EUR  310,00

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