Anti-IL-5 antibodies, mouse

Technical specifications

• Antigen: IL-5
• Clone: REA1009
• Isotype: recombinant human IgG1
• Isotype control: REA Control antibodies
• Alternative names of antigen: IL5, B-cell growth factor II, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, TRF
• Entrez Gene ID: 16191
• Molecular mass of antigen [kDa]: 13
• Cross-reactivity: human
• Distribution of antigen: T cells, T helper cells
• Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
• Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
• Storage: Store protected from light at 2–8 °C. Do not freeze.
• Available conjugates: PE, APC

• Figure 1

  Splenocytes from Balb-c mice were either left unstimulated (left images) or stimulated with 20 ng/mL PMA and 1 µg/mL ionomycin for 6 hours. After two hours, 1 µg/mL brefeldin A was added. Cells were fixed, permeabilized, and stained with Anti-IL-5 antibodies as well as with CD154 and CD4 antibodies. CD4 + cells were pre-gated for the analysis. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris were excluded from the analysis based on scatter signals.

• Figure 2

  Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with 50 ng/mL PMA and 1 µg/mL ionomycin for six hours. After two hours, brefeldin A was added. Cells were fixed, permeabilized, and intracellularly stained with Anti-IL-5 antibodies as well as with CD69 and CD4 antibodies. CD4 + cells were pre-gated for the ananlysis. Flow cytometry was performed using the MACSQuant ® Analyzer. Cell debris were excluded from the analysis based on scatter signals.

• Selected references

  1. Schwarze, J. et al. (1999) IL-5 and eosinophils are essential for the development of airway hyperresponsiveness following acute respiratory syncytial virus infection. J. Immunol. 162(5): 2997-3004
  2. Brunet, L. R. et al. (1999)
     Interleukin 5 (IL-5) is not required for expression of a Tʜ2 response or host resistance mechanisms during murine

     schistosomiasis mansoni

     but does play a role in development of IL-4-producing non-T, non-B cells.
     Infect. Immun. 67(6): 3014-3018