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Mouse spleen cells were incubated with or without (left image) PMA/Ionomycin for 6 hours. After 2 hours, brefeldin A was added. The cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-17A. Cell surface staining was performed with CD4-APC or CD4-PE. Cells were analyzed by flow cytometry. B cells and cell debris were excluded from the analysis.
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