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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left image) or stimulated with CytoStim for 6 hours. After 2 hours, brefeldin A was added. Cells were fixed, permeabilized, and stained with Anti-IL-13 antibodies and CD154 antibodies. Cells were analyzed by flow cytometry. Plots show CD4+
lymphocytes gated according to CD4-FITC staining.
Rhesus monkey PBLs were either left unstimulated (left image) or stimulated with SEB for 6 hours. After 2 hours, brefeldin A was added. Cells were fixed, permeabilized, and stained with CD4-FITC, CD154-APC, and Anti-IL-13-PE. Cells were analyzed by flow cytometry, gating was performed according to CD4 expression and scatter properties.
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