Anti-Gr-1 antibodies, mouse

Anti-Gr-1 antibodies, mouse

Clone: RB6-8C5 | Dilution: 1:10
The mouse Anti-Gr-1 antibody reacts with Ly-6G, a 21–25 kDa, GPI-anchored cell surface protein, previously defined as the granulocyte-differentiation antigen-1 (Gr-1). The antibody has been shown to cross-react weakly with Ly-6C-transfected EL-4J cells. Cross-reactivity of Gr-1 with Ly-6C was not detected on hematopoietic cells that express Ly-6C and are negative for Ly-6G.
Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues.
When using formaldehyde-fixed cells that express Gr-1 at high levels, for example, granulocytes, the dilution is 1:50 for up to 10⁷ cells/100 µL of buffer for labeling of cells and analyzing by flow cytometry.

Alternative names

Ly-6G

Technical specifications

  • Antigen: Gr-1
  • Clone: RB6-8C5
  • Isotype: rat IgG2bκ
  • Isotype control: Rat IgG2b – isotype control antibodies
  • Alternative names of antigen: Ly-6G
  • Entrez Gene ID: 546644
  • Molecular mass of antigen [kDa]: 10
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: FITC, PE, VioBlue, PerCP-Vio700
  • Selected references

    1. Hestdal, K. et al. (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J. Immunol. 147: 22-28
    2. Fleming, T. J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J. Immunol. 151: 2399-2408
    3. Nagendra, S. and Schlueter, A. J. (2004) Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A 58: 195-200
Product options: 7
9 µg in 300 µL (1)
EUR  25,00
30 µg in 1 mL (1)
EUR  40,00
9 µg in 300 µL (1)
EUR  32,00
30 µg in 1 mL (1)
EUR  70,00
9 µg in 300 µL (1)
EUR  67,00
30 µg in 1 mL (1)
EUR  110,00
30 µg in 1 mL (1)
EUR  120,00

Related products

5 products available | view all