The innovative StraightFrom REAlease Technology skips the time-consuming step of density gradient centrifugation and allows full flexibility with cell labeling. The purified target cells are bead-free, practical for consecutive magnetic isolations, and can be label-free so that epitopes are available for any downstream application. The isolated cells are suitable for flow cytometric analyses, molecular biology applications, and functional studies.
In the diagram below, we show you the steps and approximate amount of time needed for conventional, StraightFrom MicroBeads, and StraightFrom REAlease MicroBeads protocols.
Conventional protocols require the isolation of PBMCs by density gradient centrifugation before target cell isolation. The innovative StraightFrom REAlease Technology omits this time-consuming step delivering bead-free target cells directly from blood materials within 40 minutes.
The protocol for StraightFrom REAlease MicroBeads is based on positive isolation and the use of REAlease Biotin Complex.
The MultiMACS Cell24 Separator Plus is designed to process an entire buffy coat in a single run with StraightFrom® Buffy Coat REAlease® MicroBead kit. The 40-minute isolation procedure results in bead-free cell populations with high purity and recovery rates. With 12 Whole Blood Columns (included in the kit), the instrument allows time saving cell isolation when processing large sample volumes, while providing reliability and reproducibility in day-to-day cell separations.
For fully automated cell separations, the MultiMACS X is the instrument to choose.