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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Mouse skeletal muscle was dissociated using the Skeletal Muscle Dissociation Kit, mouse and rat (# 130-098-305) in combination with the gentleMACS Dissociator. Subsequently, satellite cells were isolated using the Satellite Cell Isolation Kit, mouse and cultured in expansion medium (40% DMEM, 40% HAM’s F10, 2.5 ng/mL human FGF-2, 100 U/mL penicillin/ 100 U/mL streptomycin) for six days.
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