User
Favorite list
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
EpCAM + human breast carcinoma cells were isolated from a heterogeneous sample of human cells using the Tumor Cell Isolation Kit, human, an LS Column, and a QuadroMACS™ Separator after the tissue was dissociated using the Tumor Dissociation Kit, human and the gentleMACS™ Octo Dissociator. The cells were fluorescently stained with CD326 (EpCAM)‑VioBlue ® and CD31‑PE and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Before separation | Isolated human tumor cells |
 |  |
EpCAM positive human breast carcinoma cells were isolated from a heterogeneous sample of human cells using the Tumor Cell Isolation Kit, human, an LS Column, and a QuadroMACS™ Separator. Subsequently, the unseparated fraction and the negative fraction were cultured for seven days in expansion medium (90% RPMI-1640, 2mM L-Glutamine, 10% FBS, 100 U/mL penicillin, and 100 U/mL streptomycin). After fixation, cells were stained using antibodies specific for vimentin (red), labeling fibroblasts, and EpCAM (green), labeling tumor cells. Cell nuclei were stained with DAPI (blue). |
Before separation | Isolated human tumor cells |
 |  |
Copyright © 2019 Miltenyi Biotec and/or its affiliates. All rights reserved.
