CD45RO MicroBeads, human

CD45RO MicroBeads, human

CD45RO MicroBeads were developed for positive selection or depletion of lymphocyte subsets from human PBMCs.

Background information

CD45RO is expressed on CD4
+
and CD8
+
T memory cells as well as on CD4
+
effector T cells. CD45RO is also expressed on monocytes, macrophages, and granulocytes. The CD45RO antibody recognizes a 180 kDa isoform of the leukocyte common antigen (LCA).
CD45RO MicroBeads can be used for positive selection or depletion of CD45RO
+
cells. For isolation of CD45RO
+
T cell subsets, the CD45RO MicroBeads can be combined with CD4 and CD8 MACS® T Cell Isolation Kits
1,2,7,8
or MultiSort Kits
3,4
. The CD45RO MicroBeads can also be used to isolate CD45RA
+
cells from pre-sorted CD4
+
or CD8
+
T cells by depletion of CD45RO
+
cells.
5,7,8

Downstream applications

Cells isolated with CD45RO MicroBeads can be used for various studies, such as cytokine expression
6
or receptor signaling.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.
  • Selected references

    1. Krug et al. (2001) Toll-like receptor expression reveals CpG DNA as a unique microbial stimulus for plasmacytoid dendritic cells which synergizes with CD40 ligand to induce high amounts of IL-12. Eur. J. Immunol. 31: 3026-3037
    2. Coccia, E. M. et al. (1999) Interleukin-12 induces expression of interferon regulatory factor-1 via signal transducer and activator of transcription-4 in human T helper type 1 cells. J. Biol. Chem. 274: 6698-6703
    3. Wolthers, K. C. et al. (1999) Normal T-cell telomerase activity and upregulation in human immunodeficiency virus-1 infection. Blood 93: 1011-1019
    4. Jonuleit et al. (2001)
      Identification and functional characterization of human CD4
      +
      CD25
      +
      T cells with regulatory properties isolated from peripheral blood.
      J. Exp. Med. 193: 1285-1294
    5. Kimmig, S. et al. (2002) Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J. Exp. Med. 195: 789-794
    6. Malmberg, K. J. et al. (2001)
      Inhibition of activated/memory (CD45RO
      +
      ) T cells by oxidative stress associated with block of NF-kappaB activation.
      J. Immunol. 167: 2595-2601
    7. Wagner, M. et al. (2004) IL-12p70-dependent Th1 induction by human B cells requires combined activation with CD40 ligand and CpG DNA. J. Immunol. 172: 954-963
    8. Verhasselt et al. (2004) Induction of FOXP3-expressing regulatory CD4pos T cells by human mature autologous dendritic cells. Eur. J. Immunol. 34: 762-772
Product options: 1
for 1×
10
9
total cells
EUR  612,00

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