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Isolation of CD4+
RTEs from PBMCs using the CD4+
Recent Thymic Emigrant Isolation Kit, an LS Column, and an MS Column. To evaluate the isolation of CD4+
RTEs, PBMCs before separation and the isolated CD4+
RTEs were fluorescently stained with CD4, CD45RA, and CD31 antibodies and analyzed by flow cytometry.
PBMCs before separation
PBMCs before separation gated on CD4+
RTEs gated on CD4+
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