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regulatory T cells were isolated from mouse spleen cell suspension by using the CD4+
Regulatory T Cell Isolation Kit, an LD and two MS Columns, a MidiMACS™ Separator and a MiniMACS™ Separator. The cells were fluorescently stained with CD25-PE and CD4‑FITC (A) or Anti-FoxP3-APC (B) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from analysis based on scatter signals and propidium iodide fluorescence.
CD4+ CD25- T cells
Isolated CD4+ CD25+ regulatory T cells
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