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Isolation of CD90.1+
RGCs from postnatal day 7 rat retinal tissue using the Neural Tissue Dissociation Kit - Postnatal Neurons, the Retinal Ganglion Cell Isolation Kit, a MACSiMAG Separator, an OctoMACS™ Separator, and MS Columns. Cells were fluorescently stained with CD48-PE and CD90.1-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
retinal ganglion cells
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