Basophil Isolation Kit II, human

Basophil Isolation Kit II, human

The Basophil Isolation Kit II is an indirect magnetic labeling system for the negative isolation of basophils from PBMCs.

Detailed separation procedure

Non-basophils (i.e. T cells, NK cells, B cells, monocytes, dendritic cells, erythroid cells, platelets, neutrophils, and eosinophils) are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure basophils is achieved by depletion of the magnetically labeled cells.

Downstream applications

Isolated basophils have been used for phenotypical characterization
3
, for histamine, leukotriene, and cytokine
1, 4, 5, 6
release assays, for molecular studies
1, 2, 5
, protein analysis
4, 5
, and other applications.

Columns

LS, XS, or autoMACS
®
Columns.
  • Selected references

    1. Shiratori, I. et al. (2005) Down-regulation of basophil function by human CD200 and human herpesvirus-8 CD200. J. Immunol. 175: 4441-4449
    2. Nishiyama, C. et al. (2004) Polymorphisms in the Fc epsilon RI beta promoter region affecting transcription activity: a possible promoter-dependent mechanism for association between Fc epsilon RI beta and atopy. J. Immunol. 173: 6458-6464
    3. de Paulis, A. et al. (2004) Urokinase induces basophil chemotaxis through a urokinase receptor epitope that is an endogenous ligand for formyl peptide receptor-like 1 and -like 2. J. Immunol. 173: 5739-5748
    4. Gibbs, B. F. et al. (2002) Regulation of mediator secretion in human basophils by p38 mitogen-activated protein kinase: phosphorylation is sensitive to the effects of phosphatidylinositol 3-kinase inhibitors and calcium mobilization. J. Leukoc. Biol. 72: 391-400
    5. Lavens-Phillips, S.E. et al. (2000) The tyrosine kinases p53/56lyn and p72syk are differentially expressed at the protein level but not at the messenger RNA level in nonreleasing human basophils. Am. J. Respir. Cell Mol. Biol. 23: 566-571
    6. Haisch, K. et al. (1999) Purification of morphologically and functionally intact human basophils to near homogeneity. J. Immunol. Methods 226: 129-137
Product options: 1
for 2×
10
9
total cells
EUR  901,00

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