Human IL-8

Human IL-8

IL-8 stands for interleukin 8. Human IL-8 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-8 can be used for a variety of applications, including:
  • Study of leukocyte chemotaxis.
  • Investigation of CXCR1 and CXCR2 signaling pathway.

Background information

Interleukin 8 (IL-8), also known as CXCL8, is a pro-inflammatory molecule belonging to the CXC chemokine family. The precursor form of IL-8 is cleaved into several mature isoforms (including 72 aa and 77 aa) and is secreted by several type of cells, especially monocytes and endothelial cells, upon pro-inflammatory stimulation. IL-8 signals through the CXCR1 and CXCR2 receptors, chemoattracts and activates granulocytes, predominantly neutrophils, T cells, and plays also an angiogenic role. Overexpression of IL-8 can lead to epithelial damage and injury of the airway tissues.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Migration assay using neutrophils (NIBSC code 89/520)
  • premium grade: ≥ 1×
    10
    6
    IU/mg
  • research grade: ≥ 8×
    10
    5
    IU/mg
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
EUR  100,00
E. coli
25 µg
EUR  155,00
E. coli
10 µg
EUR  130,00
E. coli
25 µg
EUR  190,00
E. coli
100 µg
EUR  525,00
E. coli
1000 µg
EUR  2.220,00