Clone:
REA146
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
LAT1, pp36

Specifications for LAT pY226 Antibody, anti-human, REAfinity™

Overview

Clone REA146 recognizes LAT phosphorylated at tyrosine 226. Linker of T cell activation (LAT), a transmembrane adaptor molecule, is constitutively localized in the lipid rafts and is involved in transducing the signal initiated by triggered T cell receptor (TCR). Upon TCR engagement, SRC and SYK family of protein tyrosine kinases (PTKs) are activated and LAT is phosphorylated at multiple conserved tyrosine residues in its cytosolic domain by Syk-family PTK Zap-70. Phosphorylated LAT can associate with several downstream SH2 domain containing proteins such as GRB2, PLCy1, ITK, GADS, and p85 subunit of PI3K to propagate the TCR activation signal and to activate several downstream pathways. pY226 motif can be bound directly by GRB2, GADS, and PLC-y1. Expression of LAT is found in thymocytes, T cells, mast cells, natural killer (NK cells), and platelets. Thymocytes lacking LAT are completely blocked at the pro-T3 stage in thymopoiesis.
Additional information: Clone REA146 displays negligible binding to Fc receptors.

Alternative names

LAT1, pp36

Detailed product information

Technical specifications

CloneREA146
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenLAT pY226
Alternative names of antigenLAT1, pp36
Molecular mass of antigen [kDa]28
Distribution of antigenT cells, NK cells, mast cells, platelets
Entrez Gene ID27040
RRIDAB_2652597, AB_2652598, AB_2652599, AB_2652600, AB_2652601, AB_2652602, AB_2652603, AB_2652596

References for LAT pY226 Antibody, anti-human, REAfinity™

Publications

  1. Finco, T. S. et al. (1998) LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway. Immunity 9(5): 617-626
  2. Zhang, W. et al. (1999) Essential role of LAT in T cell development. Immunity 10(3): 323-332
  3. Zhang, W. et al. (1998) LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation. Cell 92(1): 83-92

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