Clone:
REA718
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
integrin b5, integrin beta 5, ITGB5

Extended validation for Integrin β5 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of Integrin β5 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with Integrin β5-PE (REA718, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of Integrin β5 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with Integrin β5-PE (REA718, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of Integrin β5 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with Integrin β5-PE (REA718, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of Integrin ß5 knockout cells. Wild type (red) and knockout cells (blue) were stained with Integrin ß5-PE, clone (REA718). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of Integrin ß5 knockout cells. Wild type (red) and knockout cells (blue) were stained with Integrin ß5-PE, clone (REA718). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Integrin β5 Antibody, anti-human, REAfinity™

Overview

Clone REA718 recognizes the human integrin β5 antigen, a 95 kDa glycoprotein single-pass type I membrane protein. Integrins are a family of transmembrane receptors that mediate adhesion of cells to extracellular matrices, as well as intercellular interactions. These interactions transduce signals that control complex cell functions such as proliferation, differentiation, and survival, and require the regulation of gene expression. Integrins are heterodimeric glycoprotein receptors and exist as non-covalently bound α and β subunits. The integrin αV/β5 heterodimer is found on many types of tissue cells, such as epithelial cells, endothelial cells, keratinocytes, and osteoblastic cells. It is a receptor for fibronectin and vitronectin and acts as a receptor for adenovirus type C.
Additional information: Clone REA718 displays negligible binding to Fc receptors.

Alternative names

integrin b5, integrin beta 5, ITGB5

Detailed product information

Technical specifications

CloneREA718
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenIntegrin β5
Alternative names of antigenintegrin b5, integrin beta 5, ITGB5
Molecular mass of antigen [kDa]86
Distribution of antigenendothelial cells, epithelial cells, keratinocytes, osteoblasts
Entrez Gene ID3693
RRIDAB_2652481, AB_2652482, AB_2652483, AB_2652484, AB_2652485, AB_2652486, AB_2652487, AB_2652488, AB_2652489, AB_2652490, AB_2652491, AB_2652480

Resources for Integrin β5 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Integrin β5 Antibody, anti-human, REAfinity™

Publications

  1. Kim, J. P. et al. (1994) Vitronectin-driven human keratinocyte locomotion is mediated by the alpha v beta 5 integrin receptor. J. Biol. Chem. 269(43): 26926-26932
  2. Zhang, H. et al. (2004) Myosin-X provides a motor-based link between integrins and the cytoskeleton. Nat. Cell Biol. 2004(6): 523-531
  3. Lyle, C. et al. (2010) Integrin alphavbeta5 is a primary receptor for adenovirus in CAR-negative cells. J. Virol. 7(148): 1743-1745

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