The IL-13 Secretion Assay was developed for the sensitive detection of human IL-13-secreting cells.

Data and images for IL-13 Secretion Assay – Detection Kit (PE), human

Figures

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, cells were analyzed for IL-13 secretion as well as CD154 expression using the IL-13 Secretion Assay - Detection Kit, and CD154-APC. The plots show IL-13 secretion and CD154 expression by viable CD4
+
T cells.
Stimulated sample
Unstimulated control
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, cells were analyzed for IL-13 secretion as well as CD154 expression using the IL-13 Secretion Assay - Detection Kit, and CD154-APC. The plots show IL-13 secretion and CD154 expression by viable CD4
+
T cells.
View details

Figure 1

PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, cells were analyzed for IL-13 secretion as well as CD154 expression using the IL-13 Secretion Assay - Detection Kit, and CD154-APC. The plots show IL-13 secretion and CD154 expression by viable CD4
+
T cells.

Specifications for IL-13 Secretion Assay – Detection Kit (PE), human

Overview

The IL-13 Secretion Assay was developed for the sensitive detection of human IL-13-secreting cells.

Detailed product information

Background information

Interleukin-13 (IL-13) is produced primarily by activated Th2 cells, but also by mast cells, NK cells, and dendritic cells. IL-13 exerts anti-inflammatory effects on monocytes and macrophages. Furthermore, it inhibits the expression of pro-inflammatory cytokines, such as IL-1β, TNF-α, IL-6, and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in an increased production of IgE.
IL-13, in contrast to IL-4, fails to induce Th2 cell differentiation, one of the hallmarks of the allergic response.

Resources for IL-13 Secretion Assay – Detection Kit (PE), human

Related products for
IL-13 Secretion Assay – Detection Kit (PE), human

2 products available