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Splenocytes from C57BL/6 mice, either left unlabeled (left image) or labeled with anti-mouse CD16/CD32 antibodies (isotype rat IgG2b), were stained with Anti-IgG2b antibodies as well as with CD45R (B220) antibodies. The FcR Blocking Reagent has been used to avoid Fc receptor-mediated antibody labeling. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Clone | RG7/11.1 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG2bκ |
Isotype control | Isotype Control Antibody, mouse IgG2b |
Host | mouse |
Type of antibody | Secondary antibodies |
Species | rat |
Antigen | IgG2b |
Molecular mass of antigen [kDa] | 37 |
Distribution of antigen | B cells |
RRID | AB_2661507, AB_2661508, AB_2661509, AB_2661510, AB_2661511, AB_2661512, AB_2661513, AB_2661514, AB_2661515, AB_2661516, AB_2661517, AB_2661518, AB_2661519, AB_2661520, AB_2889692 |
Clone | RG7/11.1 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG2bκ |
Isotype control | Isotype Control Antibody, mouse IgG2b |
Host | mouse |
Type of antibody | Secondary antibodies |
Species | rat |
Antigen | IgG2b |
Molecular mass of antigen [kDa] | 37 |
Distribution of antigen | B cells |
RRID | AB_2661507, AB_2661508, AB_2661509, AB_2661510, AB_2661511, AB_2661512, AB_2661513, AB_2661514, AB_2661515, AB_2661516, AB_2661517, AB_2661518, AB_2661519, AB_2661520, AB_2889692 |
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