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Our local employees are always happy to answer your questions. Highly trained and experienced teams in your country can provide quick, helpful, and comprehensive support.
Miltenyi Biotec S.r.l.
Via Paolo Nanni Costa, 30
40133 Bologna
Italy
Phone: +39 051 6 460 411
Fax: +39 051 6 460 499
E-Mail: macs@miltenyibiotec.it
Web: www.miltenyibiotec.com
For immediate technical support, use our live chat. Connect with us
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left images) or stimulated with SEB for six hours. After two hours, brefeldin A was added for stimulated and unstimulated cells. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ antibodies as well as with CD4 antibodies and CD69 antibodies. Cells were then analyzed by flow cytometry using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. CD4+ lymphocytes were pre-gated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
Clone | 45-15 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG1κ, mouse IgG1 |
Isotype control | Isotype Control Antibody, mouse IgG1 |
Host | mouse |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | human, non-human primate |
Cross-reactivity | rhesus monkey ( Macaca mulatta) cynomolgus monkey ( Macaca fascicularis) |
Antigen | IFN-γ |
Alternative names of antigen | IFNg, IFG, IFI |
Molecular mass of antigen [kDa] | 16 |
Entrez Gene ID | 3458 |
RRID | AB_2733589, AB_2733718, AB_2733719, AB_2751182, AB_2751116, AB_2751183, AB_2751117, AB_2733079, AB_2733080, AB_10830868, AB_2733588 |
Clone | 45-15 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG1κ, mouse IgG1 |
Isotype control | Isotype Control Antibody, mouse IgG1 |
Host | mouse |
Type of antibody | Primary antibodies, Functional-grade antibodies |
Species | human, non-human primate |
Cross-reactivity | rhesus monkey ( Macaca mulatta) cynomolgus monkey ( Macaca fascicularis) |
Antigen | IFN-γ |
Alternative names of antigen | IFNg, IFG, IFI |
Molecular mass of antigen [kDa] | 16 |
Entrez Gene ID | 3458 |
RRID | AB_2733589, AB_2733718, AB_2733719, AB_2751182, AB_2751116, AB_2751183, AB_2751117, AB_2733079, AB_2733080, AB_10830868, AB_2733588 |
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