Human IL-4

Name: Human IL-4
Availability: InStock

Human IL-4

Recombinant human IL-4 (interleukin 4) can be used for T

_H

2 differentiation of T cells and stimulation of B cells. The pleiotropic cytokine is associated with allergies and asthma, as it plays a central role in humoral and adaptive immune responses. The recombinant protein IL-4 is optimized for use in functional assays, differentiation studies, and cell culture.

Product data and images for Human IL-4

Figures

Figure 1

**Human IL-4 activity assay.**
The biological activity of Human IL-4, premium grade was determined by proliferation assay using TF-1 cells.

Figure 1

Human IL-4 activity assay.

The biological activity of Human IL-4, premium grade was determined by proliferation assay using TF-1 cells.

Figure 2

**SDS-PAGE of Human IL-4, premium grade**
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human IL-4, premium grade

under reduced (R) and non-reduced (NR) conditions.

Figure 3

**Human IL-4 biological activity.**
Activity of Human IL-4, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 3

Human IL-4 biological activity.

Activity of Human IL-4, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 4

Mass spectrometry analysis (ESI-MS) of Human IL-4, premium grade. The peak corresponds to the calculated molecular mass of 15088 Da.

Figure 4

Mass spectrometry analysis (ESI-MS) of Human IL-4, premium grade. The peak corresponds to the calculated molecular mass of 15088 Da.

Specifications for Human IL-4

Overview

Recombinant human IL-4 (interleukin 4) can be used for T

_H

2 differentiation of T cells and stimulation of B cells. The pleiotropic cytokine is associated with allergies and asthma, as it plays a central role in humoral and adaptive immune responses. The recombinant protein IL-4 is optimized for use in functional assays, differentiation studies, and cell culture.

Applications

Human IL-4 can be used for a variety of applications including:

In vitro generation of Mo-DCs together with GM-CSF.
In vitro differentiation of naive CD4⁺ T cells towards T_H2 cells.

Detailed product information

Background information

Interleukin 4 (IL-4) is a pleiotropic cytokine and plays a central role in humoral and adaptive immune responses. IL-4 is predominantly secreted by activated CD4

⁺

memory and effector T

_H

2 cells, basophils, and mast cells. It promotes the proliferation and differentiation of B cells, as well as immunoglobulin isotype switching, and MHC class II antigen and low-affinity IgE receptor expression. Furthermore, IL-4 induces the differentiation of naive CD4

⁺

T cells into helper T

_H

2 cells, while suppressing T

_H

1 development, and promotes chemotaxis of mast cells and basophils. Excessive IL-4 production and mechanisms involving T

_H

2 types have been associated with immunological disorders, such as IgE-mediated allergy.

Biological activity

Proliferation of TF-1 cells (NIBSC 88/656)
research grade: ≥ 2×
10
⁶
IU/mg
premium grade: ≥ 5×
10
⁶
IU/mg

(Typical specific activity: ≥ 7.5×
10
⁶
IU/mg
)
We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human IL-4

Documents and Protocols

Background information

Premium-grade cytokine benefits

App notes/customer reports

From monocyte isolation to DC characterization - Generation of Mo-DCs

App notes/customer reports

How to switch to optimal activity-based cytokine dosing

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for Human IL-4

Publications

Kitamura, T. et al. (1991) IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoetic cell line, TF-1. Int. Immunol. 3: 571-577
Ackaert, C. et al. (2021) Immunogenicity Risk Profile of Nanobodies. Front Immunol 12: 632687
Thépaut, M. et al. (2021) DC/L-SIGN recognition of spike glycoprotein promotes SARS-CoV-2 trans-infection and can be inhibited by a glycomimetic antagonist. PLoS Pathog. 17(5): e1009576
Feray, A. et al. (2020) How to Address the Adjuvant Effects of Nanoparticles on the Immune System. Nanomaterials (Basel) 10(3): 425
Foord, E. et al. (2020) Profound Functional Suppression of Tumor-Infiltrating T-Cells in Ovarian Cancer Patients Can Be Reversed Using PD-1-Blocking Antibodies or DARPin® Proteins. J Immunol Res : 7375947
Giroud, P. et al. (2020) Expression of TAM-R in Human Immune Cells and Unique Regulatory Function of MerTK in IL-10 Production by Tolerogenic DC. Front Immunol 11: 564133
Novak, N. et al. (2020) Interaction of Monocyte-Derived Dendritic Cells with Ara h 2 from Raw and Roasted Peanuts. Foods 9(7): 863
Zanoni, M. et al. (2020) Innate, non-cytolytic CD8+ T cell-mediated suppression of HIV replication by MHC-independent inhibition of virus transcription. PLoS Pathog. 16(9): e1008821
De Laere, M. et al. (2018) Shuttling Tolerogenic Dendritic Cells across the Blood-Brain Barrier In Vitro via the Introduction of De Novo C-C Chemokine Receptor 5 Expression Using Messenger RNA Electroporation. Front Immunol 8: 1964
Johnson, M. J. et al. (2018) Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism. Sci Rep 8(1): 12144
Hsieh, S.-H. et al. (2017) Persistence within dendritic cells marks an antifungal evasion and dissemination strategy of Aspergillus terreus. Sci Rep 6;7(1): 10590
Koliha, N. et al. (2016) Melanoma affects the composition of blood cell-derived extracellular vesicles. Front Immunol 7: 282
Perriard, G. et al. (2015) Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes. J. Neuroinflammation 12: 119
Sampangi, S. et al. (2015) The Mechanisms of Human Renal Epithelial Cell Modulation of Autologous Dendritic Cell Phenotype and Function. PLoS One 10(7): e0134688
Bacher, P. et al. (2014) Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol 7(4): 916-928
Grötsch, B. et al. (2014) The AP-1 transcription factor Fra1 inhibits follicular B cell differentiation into plasma cells. J. Exp. Med. 211(11): 2199-2212
Herr, F. et al. (2014) IL-2 phosphorylates STAT5 to drive IFN-γ production and activation of human dendritic cells. J Immunol 192: 5660-5670
Kaebisch R. et al. (2014) Helicobacter pylori cytotoxin-associated gene A impairs human dendritic cell maturation and function through IL-10-mediated activation of STAT3. J Immunol 192: 316-323
Guery, L. et al. (2014) Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118: 4694-4704
Kassianos, A. J. et al. (2013) Human proximal tubule epithelial cells modulate autologous dendritic cell function. Nephrol Dial Transplant 28: 303-312
Klatka, J. et al. (2013) Expression of selected regulatory molecules on the CD83+ monocyte-derived dendritic cells generated from patients with laryngeal cancer and their clinical significance. Eur. Arch. Otorhinolaryngol. 270(10): 2683-2693
Chase A. J. et al. (2011)
Impairment of CD4
⁺
T cell polarization by dengue virus-infected dendritic cells.
J. Infect. Dis. 203: 1763-1774
Zhu, J. et al. (2006) Gfi-1 plays an important role in IL-2-mediated Tʜ2 cell expansion. Proc. Natl. Acad. Sci. U.S.A. 103: 18214-18219
Kandler, K. et al. (2006) The anti-microbial peptide LL-37 inhibits the activation of dendritic cells by TLR ligands. Int. Immunol. 18: 1729-1736

Data and images