Alternative names:
basic FGF

Data and images for Human FGF-2 IS

Figures

Figure 1

View details
Human FGF-2 IS activity assay.
The biological activity of Human FGF-2 IS, premium grade, was determined by proliferation assay using 3T3 cells. Activity of Human FGF-2 IS, premium grade, (red line) was compared to wild type Human FGF-2 (black line), and another commercially available product (gray line).

Figure 1

Human FGF-2 IS activity assay.
The biological activity of Human FGF-2 IS, premium grade, was determined by proliferation assay using 3T3 cells. Activity of Human FGF-2 IS, premium grade, (red line) was compared to wild type Human FGF-2 (black line), and another commercially available product (gray line).

Figure 2

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Thermostability of Human FGF-2 IS.
Activity of Human FGF-2 IS, premium grade (red line) versus wild type Human FGF-2 (black line), and another commercially available product (gray line), over 5 days at 37 °C.

Figure 2

Thermostability of Human FGF-2 IS.
Activity of Human FGF-2 IS, premium grade (red line) versus wild type Human FGF-2 (black line), and another commercially available product (gray line), over 5 days at 37 °C.

Figure 3

View details
Thermostability of Human FGF-2 IS.
Relative activity of Human FGF-2 IS, premium grade (red line) versus wild type Human FGF-2 (black line).

Figure 3

Thermostability of Human FGF-2 IS.
Relative activity of Human FGF-2 IS, premium grade (red line) versus wild type Human FGF-2 (black line).

Figure 4

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SDS-PAGE of Human FGF-2 IS, premium grade
(Human FGF-2 IS) versus wild type Human FGF-2 (Human FGF-2), with or without trypsin treatment.

Figure 4

SDS-PAGE of Human FGF-2 IS, premium grade
(Human FGF-2 IS) versus wild type Human FGF-2 (Human FGF-2), with or without trypsin treatment.

Specifications for Human FGF-2 IS

Overview

FGF-2 IS stands for fibroblast growth factor 2 "Improved Sequence", also termed fibroblast growth factor basic (FGF-b) or basic FGF. Human FGF-2 IS is a variant of Human FGF-2 with a proprietary amino acid substitution. Human FGF-2 IS is covered by patent nos. US10,336,799 and EP2930181B1. Human FGF-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human FGF-2 can be used for a variety of applications, including:
  • Stimulation of proliferation and differentiation of several cell types, such as mesenchymal stromal cells, neural cells, and endothelial cells.
  • Long-term maintenance and propagation of undifferentiated embryonic and induced pluripotent stem cells.
  • Differentiation of neural cells starting from embryonic and induced pluripotent stem cell cultures.

Alternative names

basic FGF

Detailed product information

Background information

Fibroblast growth factor 2 (FGF-2), also termed fibroblast growth factor basic (FGF-b) or basic FGF (bFGF), belongs to the FGF family. It functions as a wide-spectrum mitogenic, angiogenic, and neurotrophic factor and stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells. FGF-2 has been implicated in a multitude of physiological and pathological processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF-2 IS is an engineered FGF-2 variant with increased thermostability and higher resistance to proteases, and retains the same biological properties as naturally occurring FGF-2.

Biological activity

  • Proliferation of 3T3 cells (NIBSC 90/712)
  • premium grade: ≥ 2×
    10
    6
    IU/mg
  • research grade: ≥ 1×
    10
    6
    IU/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Resources for Human FGF-2 IS

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human FGF-2 IS

Publications

  1. Robinson, C. J. and Gaines-Das, R. (1994)
    The international standard for basic fibroblast growth factor (FGF-2); comparison of candidate preparations by
    in vitro
    bioassays and immunoassays.
    Growth Factors 11: 9-16