Human Activin A is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Data and images for Human Activin A

Figures

Figure 1

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Human Activin A activity assay.
The biological activity of Human Activin A, premium grade was determined by inhibition assay using MPC-11 cells.

Figure 1

Human Activin A activity assay.
The biological activity of Human Activin A, premium grade was determined by inhibition assay using MPC-11 cells.

Figure 2

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SDS-PAGE of Human Activin A, premium grade
under reducing (R) and non-reducing (NR) conditions.

Figure 2

SDS-PAGE of Human Activin A, premium grade
under reducing (R) and non-reducing (NR) conditions.

Figure 3

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Mass spectrometry analysis (ESI-MS) of Human Activin A, premium grade. The peak corresponds to the calculated molecular mass of 25934 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Human Activin A, premium grade. The peak corresponds to the calculated molecular mass of 25934 Da.

Specifications for Human Activin A

Overview

Human Activin A is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human Activin A can be used for a variety of applications, including:
  • Maintenance of pluripotency of embryonic stem cells under feeder- and serum-free conditions.
  • Differentiation of embryonic stem cells and iPS cells.
  • Stimulation and differentiation of mesenchymal cells.

Detailed product information

Background information

Activin A is a member of the TGF-β superfamily and is involved in a wide range of biological processes like growth and differentiation of several tissues. It has been described to affect embryogenesis and hematopoiesis and is necessary for the maintenance of self-renewal and pluripotency of human embryonic stem cells (hESCs). It supports long-term feeder and serum-free growth of hESCs. Activin A induces the expression of Oct4, Nanog, Nodal, Wnt3, FGF-2, and FGF-8 and suppresses the BMP signal. It also controls the expression and secretion of hormones like follicle stimulating hormone (FSH), prolactin, and ACTH (corticotropin). The amino acid sequence of human, mouse, and rat activin A shares 100% identity.

Biological activity

  • Inhibition assay using MPC-11 cells (NIBSC 91/626)
  • research grade: ≥ 0.4×
    10
    3
    U/mg
  • premium grade: ≥ 0.6×
    10
    3
    U/mg
  • We measure the biological activity of each batch of MACS Premium-Grade Cytokines and state the results in the Certificate of Analysis (CoA). Based on the lot-specific activity, exact doses of active cytokine can be applied to cell culture experiments. This allows for reproducible cell culture conditions without the need for time-consuming lot-to-lot testing.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human Activin A

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human Activin A

Publications

  1. Giacomelli, E. et al. (2020) Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease Cell Stem Cell 26(6): 862-879
  2. Leuning, D. G. et al. (2019) Vascular bioengineering of scaffolds derived from human discarded transplant kidneys using human pluripotent stem cell-derived endothelium Am. J. Transplant. 19(5): 1328-1343
  3. Jacquet, L. et al. (2015) Three Huntington's Disease Specific Mutation-Carrying Human Embryonic Stem Cell Lines Have Stable Number of CAG Repeats upon In Vitro Differentiation into Cardiomyocytes PLoS One 10(5): e0126860
  4. Gaignerie, A. et al. (2018) Urine-derived cells provide a readily accessible cell type for feeder-free mRNA reprogramming. Sci Rep (8): 14363
  5. Rodriguez-Polo, I. et al. (2021) A piggyBac-based platform for genome editing and clonal rhesus macaque iPSC line derivation Sci Rep 11(1): 15439
  6. Gandhi, J. K. et al. (2019) Human Fibrinogen for Maintenance and Differentiation of Induced Pluripotent Stem Cells in Two Dimensions and Three Dimensions Stem Cells Transl Med 8(6): 512-14521
  7. Cao, X. et al. (2019) Differentiation and Functional Comparison of Monocytes and Macrophages from hiPSCs with Peripheral Blood Derivatives Stem Cell Reports 12(6): 1282-1297
  8. Tiemeier, G. L et al. (2019) Closing the Mitochondrial Permeability Transition Pore in hiPSC-Derived Endothelial Cells Induces Glycocalyx Formation and Functional Maturation Stem Cell Reports 13(5): 803-816
  9. Phillips, D. J. et al. (1999)
    A sensitive and specific
    in vitro
    bioassay for activin using a mouse plasmacytoma cell line, MPC-11.
    J. Endocrinol. 162(1): 111-116
  10. Si-Tayeb, K. et al. (2016) Urine‑sample‑derived human induced pluripotent stem cells as a model to study PCSK9‑mediated autosomal dominant hypercholesterolemia. Dis Model Mech 9(1): 81-90
  11. Jouni, M. et al. (2015) Toward Personalized Medicine: Using Cardiomyocytes Differentiated From Urine-Derived Pluripotent Stem Cells to Recapitulate Electrophysiological Characteristics of Type 2 Long QT Syndrome J Am Heart Assoc. 4(9): e002159
  12. Lemcke, H. et al. (2020) Quantitative Evaluation of the Sarcomere Network of Human hiPSC-Derived Cardiomyocytes Using Single-Molecule Localization Microscopy Int J Mol Sci 21(8): 2819
  13. Stauske, M. et al. (2020) Non-Human Primate iPSC Generation, Cultivation, and Cardiac Differentiation under Chemically Defined Conditions Cells 9(6): 1349

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