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Analysis of antigen-specific T cells is essential for the understanding of fundamental immunological processes in the context of immuno-oncology (IO), infectious diseases, autoimmunity as well as immune tolerance.
In immuno-oncology they facilitate discovery of neo-antigens, but are also viewed as important immunotherapy effectors. In this context, their ability, to recognize and kill cancer cells make them an ideal target for IO research.
However, working with antigen-specific T cells poses several challenges to researchers. Especially their low frequencies as rare cell population often hampers a reliable analysis. Specificity, inflammatory environment, subtype as well as detection limit and background are determining factors and thus require optimized procedures in order to facilitate a successful experiment.
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