ESAM Antibody, anti-human, REAfinity™
Clone:
REA901
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
Endothelial cell-selective adhesion molecule

Extended validation for ESAM Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
View details
Overlay histogram showing flow cytometric analysis of ESAM knockout cells. Wild type (red) and knockout cells (blue) were stained with ESAM-PE, clone (REA901). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of ESAM knockout cells. Wild type (red) and knockout cells (blue) were stained with ESAM-PE, clone (REA901). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for ESAM Antibody, anti-human, REAfinity™

Overview

Clone REA901 recognizes the human endothelial cell-selective adhesion molecule (ESAM) antigen belonging to the JAM family, which is also known as a transmembrane tight junction protein composed of a single transmembrane domain and a cytoplasmic domain. ESAM is found in a high level on endothelial cells, on activated platelets, and megakaryocytes.
Additional information: Clone REA901 displays negligible binding to Fc receptors.

Alternative names

Endothelial cell-selective adhesion molecule

Detailed product information

Technical specifications

CloneREA901
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenESAM
Alternative names of antigenEndothelial cell-selective adhesion molecule
Molecular mass of antigen [kDa]38
Distribution of antigenendothelial cells, megakaryocytes, platelets
Entrez Gene ID90952
RRIDAB_2726867, AB_2726902, AB_2726868, AB_2726903, AB_2726869, AB_2726904, AB_2726870, AB_2726905, AB_2726871, AB_2928629, AB_2928628, AB_2726900, AB_2726866, AB_2928631, AB_2928630, AB_2726901

Resources for ESAM Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for ESAM Antibody, anti-human, REAfinity™

Publications

  1. Hirata, K. et al. (2001) Cloning of an immunoglobulin family adhesion molecule selectively expressed by endothelial cells. J. Biol. Chem. 276(19): 16223-16231
  2. Nasdala, I. et al. (2002) A transmembrane tight junction protein selectively expressed on endothelial cells and platelets. J. Biol. Chem. 277(18): 16294-16303

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