CX3CR1 Antibody, anti-human, REAfinity™

Name: CX3CR1 Antibody, anti-human, REAfinity™
Availability: InStock

CX3CR1 Antibody, anti-human, REAfinity™Extended ValidationRecombinant

Clone:

REA385

Type of antibody:

Primary antibodies, Recombinant antibodies

Isotype:

recombinant human IgG1

Applications:

FC, MICS, IF, IHC, ICC, MC

Alternative names:

CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28, RBS11

Flow cytometry applications forCX3CR1 Antibody, anti-human, REAfinity™

APC
PE
Vio Bright V423

Conjugate:

APC

Recommended dilution:

1:50

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-119-678, 130-119-579

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-CX3CR1 antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD3 antibodies and analyzed by flow cytometry using the MACSQuant^®Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

MICS (MACSima Imaging Cyclic Staining) applications forCX3CR1 Antibody, anti-human, REAfinity™

APC
PE

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

PFA

Tested:

during development

Species tested:

human

Products used:

130-119-678, 130-119-579

Compatible applications:

IF, IHC, ICC

Protocols:

Protocol for MICS experiments

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

Acetone

Tested:

during development

Species tested:

human

Products used:

130-119-678, 130-119-579

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Mass cytometry applications forCX3CR1 Antibody, anti-human, REAfinity™

pure

Conjugate:

pure

Products used:

130-122-286

Extended validation for CX3CR1 Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CX3CR1 (REA385). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CX3CR1 (REA385). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CX3CR1 Antibody, anti-human, REAfinity™

Overview

Clone REA385 recognizes the human CX3C chemokine receptor 1 antigen, a 40 kDa G-protein–coupled multi-pass membrane protein also known as fractalkine receptor. CX3CR1 is expressed on natural killer (NK) cells, T cell subsets, monocytes/macrophages, dendritic cells (DCs), and some malignant epithelial cells. Binding of CX3CR1 to the membrane-bound form of the chemokine CX3CL1 promotes cell-cell adhesion, whereas the soluble form induces cell migration of CX3CR1-bearing cells such as monocytes, NK cells, T cells, DCs, and macrophages including microglia. CX3CR1 plays also an important role in the formation of transepithelial dendrites by intestinal DCs. Failure in the CX3CL1/CX3CR1 interaction may contribute to the development of several inflammatory diseases including atherosclerosis, psoriasis, rheumatoid arthritis, and experimental autoimmune myositis.

Additional information: Clone REA385 displays negligible binding to Fc receptors.

Alternative names

CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28, RBS11

Detailed product information

Technical specifications

Clone	REA385
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (S), human IgG1
Host	human cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human, non-human primate
Cross-reactivity	rhesus monkey ( Macaca mulatta ) , cynomolgus monkey ( Macaca fascicularis )
Antigen	CX3CR1
Alternative names of antigen	CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28, RBS11
Molecular mass of antigen [kDa]	40
Distribution of antigen	NK cells, T cells, dendritic cells, macrophages, monocytes, epithelial cells
Entrez Gene ID	1524
RRID	AB_2751738, AB_2801984, AB_2801963, AB_2801867, AB_2928611, AB_2928610, AB_2751798

Resources for CX3CR1 Antibody, anti-human, REAfinity™

Documents and Protocols

App notes/customer reports

Comprehensive flow cytometry analysis of chemokine receptors expressed on human natural killer cells.

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for CX3CR1 Antibody, anti-human, REAfinity™

Publications

Niess, J. H. et al. (2005)
CX
₃
CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance.
Science 307: 254-258
Imai, T. et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell 91: 521-530
Raport, C. J. et al. (1995) The orphan G-protein-coupled receptor-encoding gene V28 is closely related to genes for chemokine receptors and is expressed in lymphoid and neural tissues. Gene 163(2): 295-299

Applications

Extended validation