CX3CR1 Antibody, anti-human

Name: CX3CR1 Antibody, anti-human
Availability: InStock

CX3CR1 Antibody, anti-humanExtended Validation

Clone:

2A9-1

Type of antibody:

Primary antibodies

Isotype:

rat IgG2bκ

Applications:

FC

Alternative names:

CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28

Flow cytometry applications forCX3CR1 Antibody, anti-human

APC
Biotin
PE
PE-Vio 770

Conjugate:

APC

Recommended dilution:

1:11

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-099-618, 130-096-435

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:11)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-CX3CR1 antibodies as well as with CD16 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or DAPI fluorescence, as in the case of tandem-conjugates.

Extended validation for CX3CR1 Antibody, anti-human

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CX3CR1. Human peripheral blood mononuclear cells (PBMCs) were stained with CX3CR1 antibodies and with a suitable counterstaining. As a control, CX3CR1 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for CX3CR1 Antibody, anti-human

Overview

CX3CR1 is a transmembrane chemokine receptor with a molecular mass of 40 kDa. It binds the chemokine CX3CL1, also known as fractalkine or neurotactin. Binding of CX3CR1 to the membrane‑bound form of fractalkine promotes cell-cell adhesion, wheras the soluble form induces cell migration of CX3CR1-bearing cells such as monocytes, NK cells, T cells, dendritic cells (DCs), and macrophages including microglia.¹ CX3CR1 plays also an important role in the formation of transepithelial dendrites by intestinal DCs.² Failure in the fractalkine/CX3CR1 interaction may contribute to the development of several inflammatory diseases including atherosclerosis, psoriasis, rheumatoid arthritis, and experimental autoimmune myositis.

Alternative names

CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28

Detailed product information

Technical specifications

Clone	2A9-1
Clonality	monoclonal
Isotype	rat IgG2bκ
Isotype control	Isotype Control Antibody, rat IgG2b
Host	rat
Type of antibody	Primary antibodies
Species	human
Antigen	CX3CR1
Alternative names of antigen	CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28
Molecular mass of antigen [kDa]	40
Entrez Gene ID	1524
RRID	AB_10828443, AB_2651468, AB_10828236, AB_2651469, AB_2651470, AB_10829616, AB_2651467

Resources for CX3CR1 Antibody, anti-human

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for CX3CR1 Antibody, anti-human

Publications

Niess, J. H. et al. (2005)
CX
₃
CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance.
Science 307: 254-258
Imai, T. et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell 91: 521-530

Applications

Extended validation