CD2 antibodies

CD2 antibodies

Clone: LT2
LT2 recognizes the human CD2 antigen, a 50 kDa single-chain transmembrane glycoprotein also known as LFA-2 or receptor for sheep erythrocytes. CD2 belongs to the Ig superfamily and is involved in cell signaling and lymphocyte adhesion. The CD2 antibody reacts with 80–90 % of peripheral blood lymphocytes and more than 95 % of thymocytes. The antibody recognizes all T cells and a subset of NK cells.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis. Expression of CD2 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative names

LFA-2, SRBC-R, T11

Technical specifications

  • Antigen: CD2
  • Clone: LT2
  • Isotype: mouse IgG2b
  • Alternative names of antigen: LFA-2, SRBC-R, T11
  • Molecular mass of antigen [kDa]: 37
  • Distribution of antigen: B cells, lymphocytes, macrophages, mast cells, NK cells, T cells, thymocytes
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: VioBlue, FITC
  • Selected references

    1. Wood BL et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22
    2. Stelzer GT et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
    3. Ozwara, H. et al. (1997)
      Flow cytometric analysis on reactivity of human T lymphocyte-specific and cytokine-receptor-specific antibodies with peripheral blood mononuclear cells of chimpanzee (
      Pan troglodytes
      ), rhesus macaque (
      Macacca mulatta
      ) and squirrel monkey (
      Saimiri sciureus
      ).
      J. Med. Primatol. 26(3): 164-171
    4. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
    5. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
    6. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
    7. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
    8. Filatov, A. V. et al. (1989) A panel of monoclonal antibodies against human lymphocyte antigens. Eksp. Onkol. 11(2): 28-32
    9. Kishimoto, T et al. (eds) Leucocyte typing VI: White cell differentiation antigens. Garland Publishing Inc., New York
  • gentleMACS Protocols

  • Certificates

    Please follow this
    link
    to download the Certificate of Conformity (CoC) by lot number.
Product options: 2
170-078-001

CD2-FITC

for 100
tests
EUR  385,00
170-078-000

CD2-VioBlue

for 100
tests
EUR  475,00