Clone:
REA207
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
LILRB3, HL9, ILT5, LILRA6, LIR-3, PIRB

Extended validation for CD85a (ILT5) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA207
MKT5.1++
222821+
Cells were incubated with an excess of purified unconjugated CD85a (ILT5) (REA207) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD85a (ILT5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD85a (ILT5) antibodies and with a suitable counterstaining. As a control, CD85a (ILT5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD85a (ILT5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD85a (ILT5) antibodies and with a suitable counterstaining. As a control, CD85a (ILT5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD85a (ILT5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD85a (ILT5) antibodies and with a suitable counterstaining. As a control, CD85a (ILT5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD85a (ILT5). Human peripheral blood mononuclear cells (PBMCs) were stained with CD85a (ILT5) antibodies and with a suitable counterstaining. As a control, CD85a (ILT5) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD85a (ILT5) (REA207). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD85a (ILT5) (REA207). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD85a (ILT5) (REA207). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD85a (ILT5) Antibody, anti-human, REAfinity™

Overview

Clone REA207 recognizes the CD85a antigen, a immunoglobulin superfamily transmembrane glycoprotein also known as leukocyte immunoglobulin-like receptor member 3 (LIR-3) or immunoglobulin-like transcript 5 (ILT5). CD85a is preferentially expressed by monocytes, macrophages, dendritic cells, and granulocytes. It provides inhibitory signals through interaction with MHC class I molecules and becomes activated upon co-ligation of CD85a and immune receptors, such as CD32 and the B cell receptor. CD85a down-regulates antigen-induced B cell–activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitory motifs.
Additional information: Clone REA207 displays negligible binding to Fc receptors.

Alternative names

LILRB3, HL9, ILT5, LILRA6, LIR-3, PIRB

Detailed product information

Technical specifications

CloneREA207
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD85a (ILT5)
Alternative names of antigenLILRB3, HL9, ILT5, LILRA6, LIR-3, PIRB
Distribution of antigendendritic cells, granulocytes, macrophages
RRIDAB_2659333, AB_2659334, AB_2659335, AB_2659336, AB_2659337, AB_2659338, AB_2659339, AB_2659340, AB_2659341, AB_2659342, AB_2659343, AB_2659344, AB_2659345, AB_2659332

Resources for CD85a (ILT5) Antibody, anti-human, REAfinity™

References for CD85a (ILT5) Antibody, anti-human, REAfinity™

Publications

  1. Borges, L. et al. (1997) A family of human lymphoid and myeloid Ig-like receptors, some of which bind to MHC class I molecules. J. Immunol. 159: 5192-5196
  2. Ma, G. et al. (2011) Paired immunoglobin-like receptor-B regulates the suppressive function and fate of myeloid-derived suppressor cells. Immunity 34(3): 385-395
  3. Colonna, M. et al. (1999) Inhibitory and activating receptors involved in immune surveillance by human NK and myeloid cells. J. Leukoc. Biol. 66: 718-722

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