CD84 Antibody, anti-human

Name: CD84 Antibody, anti-human
Availability: InStock

CD84 Antibody, anti-humanExtended Validation

Clone:

MZ18-21F6

Type of antibody:

Primary antibodies

Isotype:

mouse IgG1κ

Applications:

FC

Alternative names:

Ly9b, SLAMF5, hCD84, mCD84, GR6

Flow cytometry applications forCD84 Antibody, anti-human

APC
Biotin
FITC
PE

Conjugate:

APC

Recommended dilution:

1:11

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-093-276

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:11)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with CD84 antibodies as well as with CD4 antibodies and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Extended validation for CD84 Antibody, anti-human

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with MZ18-21F6
CD84.1.21	-
REA1102	++
2G7	++
Cells were incubated with an excess of purified unconjugated CD84 (MZ18-21F6) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD84 (MZ18-21F6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD84 (MZ18-21F6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD84 Antibody, anti-human

Overview

Human CD84 is a 65–80 kDa single-chain surface glycoprotein and member of the CD2 subset of the immunoglobulin (Ig) superfamily. CD84 is expressed on granulocytes, monocytes, macrophages, B cells, platelets, antigen-presenting cells, and subsets of T cells including thymocytes and mature T cells. Homophilic ligation of CD84 or ligation with CD84 antibodies has been shown to lead to enhanced proliferation of T cells as well as IFN-γ secretion. CD84 thus serves as a co-stimulatory molecule. CD84 gene expression has also been detected in lung and kidney tissue as well as on hematopoietic progenitor cells in bone marrow.

Alternative names

Ly9b, SLAMF5, hCD84, mCD84, GR6

Detailed product information

Technical specifications

Clone	MZ18-21F6
Clonality	monoclonal
Isotype	mouse IgG1κ
Isotype control	Isotype Control Antibody, mouse IgG1
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	CD84
Alternative names of antigen	Ly9b, SLAMF5, hCD84, mCD84, GR6
Molecular mass of antigen [kDa]	36
Distribution of antigen	B cells, dendritic cells, granulocytes, macrophages, monocytes, NK cells, platelets, T cells, thymocytes
Entrez Gene ID	8832
RRID	AB_1036232, AB_1036226, AB_1036228, AB_1036230

Resources for CD84 Antibody, anti-human

Certificates

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References for CD84 Antibody, anti-human

Publications

Martin, M. et al. (2001) CD84 functions as a homophilic adhesion molecule and enhances IFN-γ secretion: adhesion is mediated by Ig-like domain 1. J Immunol 167: 3668-3676
Tangye, S. G. et al. (2003) Functional requirements for interactions between CD84 and Src homology 2 domain-containing proteins and their contribution to human T cell activation. J Immunol 171: 2485-2495
Zaiss, M. et al. (2003) CD84 expression on human hematopoietic progenitor cells. Exp. Hematol. 31: 798-805
de la Fuente, M. A. et al. (1997) CD84 leukocyte antigen is a new member of the Ig superfamily. Blood 90: 2398-2405
Romero, X. et al. (2004) Differential expression of SAP and EAT-2-binding leukocyte cell-surface molecules CD84, CD150 (SLAM), CD229 (Ly9) and CD244 (2B4). Tissue Antigens 64(2): 132-144

Applications

Extended validation