Name: CD8 (TIL) MicroBeads, mouse
Availability: InStock

Product data and images for CD8 (TIL) MicroBeads, mouse

Figures

Figure 1

A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD8

⁺

TILs were isolated from the single-cell suspension using CD8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8b-VioBlue

^®

and CD4-PE-Vio

^®

615 and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence.

Before separation

CD8

⁺

cells

View details

Figure 1

A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD8

⁺

TILs were isolated from the single-cell suspension using CD8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8b-VioBlue

^®

and CD4-PE-Vio

^®

615 and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence.

View details

Figure 1

A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD8

⁺

TILs were isolated from the single-cell suspension using CD8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8b-VioBlue

^®

and CD4-PE-Vio

^®

615 and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence.

Specifications for CD8 (TIL) MicroBeads, mouse

Overview

CD8 (TIL) MicroBeads, mouse have been optimized for the isolation of CD8

⁺

tumor-infiltrating lymphocytes from mouse tumors.

Detailed product information

Background information

CD8 (TIL) MicroBeads, mouse enable a positive selection of CD8

⁺

lymphocytes from solid mouse tumors, e.g., CT26.WT, 4T1, and B16F10 and have specifically been developed for an optimal isolation of tumor-infiltrating lymphocytes (TILs) from mouse tumors. The CD8 antigen is expressed on cytotoxic T cells.

Columns

MS Columns, LS Columns, autoMACS

^®

Columns, or Multi-24 Column Blocks.

Resources for CD8 (TIL) MicroBeads, mouse

Documents and Protocols

Brochures/posters

Tumor-infiltrating leukocytes - Optimized solutions for TIL research

Scientific posters

Optimized workflow improves the characterization of tumor-infiltrating T cells. SITC (2017)

Data and images

Data and images for CD8 (TIL) MicroBeads, mouse

Figures

Figure 1

A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD8

⁺

TILs were isolated from the single-cell suspension using CD8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8b-VioBlue

^®

and CD4-PE-Vio

^®

615 and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence.

Before separation

CD8

⁺

cells

View details

Figure 1

A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD8

⁺

TILs were isolated from the single-cell suspension using CD8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8b-VioBlue

^®

and CD4-PE-Vio

^®

615 and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence.

View details

Figure 1

A tumor induced by the B16-OVA cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD8

⁺

TILs were isolated from the single-cell suspension using CD8 (TIL) MicroBeads, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8b-VioBlue

^®

and CD4-PE-Vio

^®

615 and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and Viobility™ 405/520 Fixable Dye fluorescence.

Specifications

Specifications for CD8 (TIL) MicroBeads, mouse

Overview

CD8 (TIL) MicroBeads, mouse have been optimized for the isolation of CD8

⁺

tumor-infiltrating lymphocytes from mouse tumors.

Detailed product information

Background information

CD8 (TIL) MicroBeads, mouse enable a positive selection of CD8

⁺

Columns

MS Columns, LS Columns, autoMACS

^®

Columns, or Multi-24 Column Blocks.

Resources

Resources for CD8 (TIL) MicroBeads, mouse

Documents and Protocols

Brochures/posters

Tumor-infiltrating leukocytes - Optimized solutions for TIL research

Scientific posters

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CD8 (TIL) MicroBeads, mouse

CD8 (TIL) MicroBeads, mouse

Product overview

Product data and images for CD8 (TIL) MicroBeads, mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications for CD8 (TIL) MicroBeads, mouse

Overview

Detailed product information

Background information

Columns

Resources for CD8 (TIL) MicroBeads, mouse

Documents and Protocols

Data and images for CD8 (TIL) MicroBeads, mouse

Figures

Figure 1

Figure 1

Figure 1

Specifications for CD8 (TIL) MicroBeads, mouse

Overview

Detailed product information

Background information

Columns

Resources for CD8 (TIL) MicroBeads, mouse

Documents and Protocols

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