Cookie Settings
We use cookies to provide the best possible website experience for you. This includes cookies that are technically required to ensure a proper functioning of the website, as well as cookies which are used solely for anonymous statistical purposes, for more comfortable website settings, or for displaying personalized content. You are free to choose the categories you would like to permit. Please note that depending on your settings, the full functionality of the website may no longer be available. Further information can be found in our Privacy Statement and Cookie Statement.
Both the CD56 +CD8 + and CD56 +CD8 – NK cell subsets were isolated from human PBMCs using the CD56 +CD8 +/CD8 – NK Cell Isolation Kit. Non-NK cells were depleted using an LS Column. The enriched NK cells were magnetically labeled with CD8 MicroBeads, and CD56 +CD8 + NK cells were isolated by positive selection using an MS Column. For isolation of the CD56 +CD8 – NK cells, the wash and the flow-through fraction of the MS Column were further separated using an LD Column and collected in the flow-through. Cells were fluorescently stained with CD8-PE (clone 3G8) and CD56-APC. |
A: | B: |
PBMCs before separation | Pre-enriched NK cells after depletion of non-NK cells |
CD56 +CD8 +/CD8 - NK Cell Isolation Kit | CD56 +CD8 +/CD8 - NK Cell Isolation Kit |
C: | D: |
Isolated CD56 +CD8 + NK cells | Isolated CD56 +CD8 – NK cells |
CD56 +CD8 +/CD8 - NK Cell Isolation Kit | CD56 +CD8 +/CD8 - NK Cell Isolation Kit |
Both the CD56 +CD8 + and CD56 +CD8 – NK cell subsets were isolated from human PBMCs using the CD56 +CD8 +/CD8 – NK Cell Isolation Kit. Non-NK cells were depleted using an LS Column. The enriched NK cells were magnetically labeled with CD8 MicroBeads, and CD56 +CD8 + NK cells were isolated by positive selection using an MS Column. For isolation of the CD56 +CD8 – NK cells, the wash and the flow-through fraction of the MS Column were further separated using an LD Column and collected in the flow-through. Cells were fluorescently stained with CD8-PE (clone 3G8) and CD56-APC. |
A: | B: |
PBMCs before separation | Pre-enriched NK cells after depletion of non-NK cells |
CD56 +CD8 +/CD8 - NK Cell Isolation Kit | CD56 +CD8 +/CD8 - NK Cell Isolation Kit |
C: | D: |
Isolated CD56 +CD8 + NK cells | Isolated CD56 +CD8 – NK cells |
CD56 +CD8 +/CD8 - NK Cell Isolation Kit | CD56 +CD8 +/CD8 - NK Cell Isolation Kit |
Seems like you are coming from USA!
Do you want to visit our website in your country?
Copyright © 2023 Miltenyi Biotec and/or its affiliates. All rights reserved.