The CD56
+
CD16
+
NK Isolation Kit was developed for the isolation of CD56
dim
CD16
+
NK cells from PBMCs by sequential sorting. The CD56
dim
NK cell subset is highly cytotoxic even without stimulation, but has little to no cytokine production.

Data and images for
CD56
+
CD16
+
NK Cell Isolation Kit
, human

Figures

Figure 1

CD56
+
CD16
+
NK cells were isolated from human PBMCs using the CD56
+
CD16
+
NK Cell Isolation Kit, an LS and an MS Column, a MidiMACS™ and a MiniMACS™ Separator. Cells were fluorescently stained with CD16-FITC and CD56-PE.
A:
B:
PBMCs before separation
Enriched NK cells after depletion of non-NK cells
C:
Isolated CD56
+
CD16
+
NK cells

Specifications for
CD56
+
CD16
+
NK Cell Isolation Kit
, human

Overview

The CD56
+
CD16
+
NK Isolation Kit was developed for the isolation of CD56
dim
CD16
+
NK cells from PBMCs by sequential sorting. The CD56
dim
NK cell subset is highly cytotoxic even without stimulation, but has little to no cytokine production.

Detailed product information

Background information

NK cells represent a highly specialized lymphocyte population of the innate immune system playing an important role in early immune response.
NK cells can be divided into several subsets according to function and phenotype. CD56
+
CD16
+
NK cells represent the majority of all NK cells in the blood. They express killer immunoglobulin-like receptors (KIRs) and CD16 and, accordingly, show strong cell-mediated cytotoxicity as well as antibody-dependent cell-mediated cytotoxicity (ADCC). Unlike CD56
+
CD16
-
NK cells, they have a low cytokine production capacity.
1

Detailed separation procedure

The isolation of CD56
+
CD16
+
NK cells is performed in a two-step procedure. First, non-NK cells are labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads und subsequently depleted by separation over a MACS® Column, which is placed in the magnetic field of a MACS Separator. In the second step, the target cells are labeled with CD16 MicroBeads and isolated from the pre-enriched cell fraction by positive selection over a second column.

Downstream applications

Cells isolated using the CD56
+
CD16
+
NK Cell Isolation Kit can be used for the phenotypical and functional characterization of NK cell subsets, for the analysis of receptor expression, cytokine secretion, or cell-cell interaction with other cells of the innate and adaptive immune system.
2
Furthermore, cells can also be used to study the role of NK cells in diseases, e.g., hepatitis infection or cancer, as well as during pregnancy.

Columns

For the first magnetic separation (depletion): LS or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Resources for
CD56
+
CD16
+
NK Cell Isolation Kit
, human

Documents and Protocols

Reviews for
CD56
+
CD16
+
NK Cell Isolation Kit
, human

NK Cell Magnetic Bead Isolation Kit

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CD56+CD16+ NK Cell Isolation Kit, human (130-092-660)

Our lab studies NK cell activity in the context of HIV infection and use purified NK cells from human PBMC. This kit provides reliable isolation of NK cells from this cell source.

NK Cell Isolation Kit

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CD56+CD16+ NK Cell Isolation Kit, human (130-092-660)

Our laboratory investigates ways to improve immune responses against HIV, including enhancement of the capacity of NK cells to kill HIV-infected cell. For these studies we set up in vitro ADCC assays with NK cells against infected target cells. As such, we need to purify NK cells and the CD56+ CD16+ selection kit isolates this population at a high purity via a two step process, first depleting all non-NK cells, and then enriching for the CD56 and CD16 positive cells.

Human NK Isolation Kit

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CD56+CD16+ NK Cell Isolation Kit, human (130-092-660)

We used this kit in experiments where we were investigating Fc mediated functions of NK cells in the context of HIV infection. For this we needed to isolate NK cells from human PBMCs. This kit was used and consistently resulted in high purity and yield of human NK cells.

References for
CD56
+
CD16
+
NK Cell Isolation Kit
, human

Publications

  1. Deniz, G. et al. (2002) Human NK1 and NK2 subsets determined by purification of IFN-gamma-secreting and IFN-gamma-nonsecreting NK cells. Eur. J. Immunol. 32: 879-884
  2. Hagberg, N. et al. (2011) IFN-α production by plasmacytoid dendritic cells stimulated with RNA-containing immune complexes is promoted by NK cells via MIP-1β and LFA-1. J. Immunol. 186: 5085-5094

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CD56
+
CD16
+
NK Cell Isolation Kit
, human

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