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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Our local employees are always happy to answer your questions. Highly trained and experienced teams in your country can provide quick, helpful, and comprehensive support.
Miltenyi Biotec S.r.l.
Via Paolo Nanni Costa, 30
40133 Bologna
Italy
Phone: +39 051 6 460 411
Fax: +39 051 6 460 499
E-Mail: macs@miltenyibiotec.it
Web: www.miltenyibiotec.com
For immediate technical support, use our live chat. Connect with us
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Splenocytes from C56BL/6 mice were stained with REAlease CD54 (ICAM-1)-APC (B) or with the corresponding REAL Control (A). REAlease CD54 (ICAM-1)-APC was released using the REAlease Release Reagent (C). To show epitope accessibility after REAlease Complex release, the cells were washed with the REAlease Stop Reagent and restained with REAlease CD54 (ICAM-1)-APC (D). Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.
Immunofluorescence analysis of a mouse spleen cryosection fixed with 4% PFA. The section was stained using antigen_conjugate (green) for 10 min at room temperature (A). CD54 (ICAM-1) Antibody, anti-mouse, APC staining was released using the REAlease Release Reagent (B). Nuclei were visualized with Hoechst™ (blue).Scale bar: 30 µm.
Immunofluorescence analysis of a mouse spleen cryosection fixed with ice-cold acetone. The section was stained using CD54 (ICAM-1) Antibody, anti-mouse, APC (green) for 10 min at room temperature (A). antigen_conjugate staining was released using the REAlease Release Reagent (B). Nuclei were visualized with Hoechst™ (blue).Scale bar: 30 µm.
Clone | REAL289 |
---|---|
Clonality | monoclonal |
Isotype control | Control Antibody |
Host | human cell line |
Type of antibody | Releasable antibodies, Primary antibodies, Recombinant antibodies |
Species | mouse |
Antigen | CD54 (ICAM-1) |
Alternative names of antigen | ICAM-1, Ly-47, MALA-2 |
Distribution of antigen | monocytes, macrophages, lymphocytes, endothelial cells, granulocytes, dendritic cells |
RRID | AB_2784253, AB_2857439, AB_2751300 |
Clone | REAL289 |
---|---|
Clonality | monoclonal |
Isotype control | Control Antibody |
Host | human cell line |
Type of antibody | Releasable antibodies, Primary antibodies, Recombinant antibodies |
Species | mouse |
Antigen | CD54 (ICAM-1) |
Alternative names of antigen | ICAM-1, Ly-47, MALA-2 |
Distribution of antigen | monocytes, macrophages, lymphocytes, endothelial cells, granulocytes, dendritic cells |
RRID | AB_2784253, AB_2857439, AB_2751300 |
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