Clone:
REA181
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ITGAV, MSK8, VNRA, VTNR

Extended validation for CD51 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA181
23C6-
NKI-M9++
P1F6-
REA1099-
Cells were incubated with an excess of purified unconjugated CD51 (REA181) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD51. Human peripheral blood mononuclear cells (PBMCs) spiked with 20% human fibroblast cells Human fibroblast cells were stained with CD51 antibodies and plotted against the side scatter. As a control, CD51 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD51. Human peripheral blood mononuclear cells (PBMCs) spiked with 20% human fibroblast cells Human fibroblast cells were stained with CD51 antibodies and plotted against the side scatter. As a control, CD51 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD51. Human peripheral blood mononuclear cells (PBMCs) spiked with 20% human fibroblast cells Human fibroblast cells were stained with CD51 antibodies and plotted against the side scatter. As a control, CD51 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD51. Human peripheral blood mononuclear cells (PBMCs) spiked with 20% human fibroblast cells Human fibroblast cells were stained with CD51 antibodies and plotted against the side scatter. As a control, CD51 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD51. Human peripheral blood mononuclear cells (PBMCs) spiked with 20% human fibroblast cells Human fibroblast cells were stained with CD51 antibodies and plotted against the side scatter. As a control, CD51 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD51 (REA181). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD51 (REA181). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD51 (REA181). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD51 Antibody, anti-human, REAfinity™

Overview

Clone REA181 recognizes CD51, also known as αV integrin, which is a 140 KDa single pass type I membrane protein. CD51 forms heterodimers with β1, β3, β5, or β6 inetgrins and serves as a receptor for diverse extra cellular matrix proteins such as fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, laminin, and matrix metalloproteinase-2.
Additional information: Clone REA181 displays negligible binding to Fc receptors.

Alternative names

ITGAV, MSK8, VNRA, VTNR

Detailed product information

Technical specifications

CloneREA181
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD51
Alternative names of antigenITGAV, MSK8, VNRA, VTNR
Molecular mass of antigen [kDa]113
Distribution of antigenendothelial cells, fibroblasts, macrophages
Entrez Gene ID3685
RRIDAB_2658617, AB_2658618, AB_2658619, AB_2658620, AB_2658621, AB_2658622, AB_2658623, AB_2658624, AB_2658625, AB_2658626, AB_2658627, AB_2658616

Resources for CD51 Antibody, anti-human, REAfinity™

References for CD51 Antibody, anti-human, REAfinity™

Publications

  1. Dahm, L. M. et al. (1998) Vitronectin regulates smooth muscle contractility via alphav and beta1 integrin J. Cell. Sci. 111: 1175-1183
  2. Tuckwell, D. S. et al. (1993) Integrins: a review of their structure and mechanisms of ligand binding. Symp. Soc. Exp. Biol 47: 107-136

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