Clone:
REA686
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
VLA-5α, α5 integrin, VLA-5, Integrin alpha-5

Extended validation for CD49e Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD49e knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD49e-PE (REA686, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD49e knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD49e-PE (REA686, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD49e knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD49e-PE (REA686, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of human CD49e knockout cells. Wild type (red) and knockout cells (blue) were stained with CD49e-PE, clone (REA686). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of human CD49e knockout cells. Wild type (red) and knockout cells (blue) were stained with CD49e-PE, clone (REA686). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD49e Antibody, anti-human, REAfinity™

Overview

Clone REA686 recognizes the human CD49e antigen, also known as integrin α5 chain. Integrins are cell-surface receptors, expressed as heterodimers essential in various processes mediating intercellular or cellmatrix interaction. Integrin α5 is composed of a heavy and a light chain linked by a disulfide bond. CD49e associates non-covalently with the integrin ß1 (CD29) forming the very late activation antigen-5 (VLA5) complex which is expressed on many cell types including peripheral lymphocytes, monocytes, fibroblasts, and endothelial cells and is a receptor for fibronectin and fibrinogen but functions also as a receptor for laminin and several other extracellular matrix proteins.
Additional information: Clone REA686 displays negligible binding to Fc receptors.

Alternative names

VLA-5α, α5 integrin, VLA-5, Integrin alpha-5

Detailed product information

Technical specifications

CloneREA686
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD49e
Alternative names of antigenVLA-5α, α5 integrin, VLA-5, Integrin alpha-5
Molecular mass of antigen [kDa]110
Distribution of antigenendothelial cells, monocytes, fibroblasts, lymphocytes
Entrez Gene ID3678
RRIDAB_2658522, AB_2658523, AB_2658524, AB_2658525, AB_2658526, AB_2658527, AB_2658528, AB_2658521

Resources for CD49e Antibody, anti-human, REAfinity™

References for CD49e Antibody, anti-human, REAfinity™

Publications

  1. Riedl, E. et al. (2000)
    Functional involvement of E-cadherin in TGF-beta 1-induced cell cluster formation of
    in vitro
    developing human Langerhans-type dendritic cells.
    J. Immunol. 165(3): 1381-1386
  2. Takada, Y. et al. (1987) The very late antigen family of heterodimers is part of a superfamily of molecules involved in adhesion and embryogenesis. Proc. Natl. Acad. Sci. U.S.A. 84: 3239-3243
  3. Takagi, J. et al. (2003) Structure of integrin alpha5beta1 in complex with fibronectin. EMBO J. 22: 4607-4615
  4. Lomakina, E. B and Waugh, R. E. (2009) Adhesion between human neutrophils and immobilized endothelial ligand vascular cell adhesion molecule 1: divalent ion effects Biophys. J. 96(1): 276-284

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