Mouse CD49b (DX5) MicroBeads are used for the isolation of mouse NK cells from single-cell suspensions of lymphoid and non-lymphoid tissue, as well as from peripheral blood or body fluids.

Data and images for CD49b (DX5) MicroBeads, mouse

Figures

Figure 1

View details
Isolation of mouse NK cells from a mouse spleen cell suspension using CD49b (DX5) MicroBeads, a MiniMACS™ Separator, and an MS Column.

Figure 1

Isolation of mouse NK cells from a mouse spleen cell suspension using CD49b (DX5) MicroBeads, a MiniMACS™ Separator, and an MS Column.

Specifications for CD49b (DX5) MicroBeads, mouse

Overview

Mouse CD49b (DX5) MicroBeads are used for the isolation of mouse NK cells from single-cell suspensions of lymphoid and non-lymphoid tissue, as well as from peripheral blood or body fluids.

Detailed product information

Background information

CD49b is expressed on NK cells and a small population of T cells (CD4
+
CD3
+
TCRα/β
+
). These cells can be depleted prior to NK cell enrichment by using CD90.2 MicroBeads or CD5 (Ly-1) MicroBeads.
CD49b is less mouse strain–specific than other NK cell markers (e.g. NK1.1) and is expressed by the most common inbred mouse strains.

Downstream applications

NK cells isolated with CD49b (DX5) MicroBeads from wild-type or immunodeficient mice were used to study NK cell cytotoxicity
in vitro1
or after adoptive transfer of isolated NK cells
in vivo2
. Furthermore, isolated NK cells were used to evaluate their role in experimental mouse models of immune-mediated liver injury
3
and chronic infection
4
.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for CD49b (DX5) MicroBeads, mouse

Publications

  1. Tomasello, E. et al. (2000) Combined natural killer cell and dendritic cell functional deficiency in KARAP/DAP12 loss-of-function mutant mice. Immunity 13: 355-364
  2. Shi, F. D. et al. (2000) IL-18 directs autoreactive T cells and promotes autodestruction in the central nervous system via induction of IFN-gamma by NK cells. J. Immunol. 165: 3099-3104
  3. Mühlen, K. A. et al. (2004) NK cells, but not NKT cells, are involved in Pseudomonas aeruginosa exotoxin A-induced hepatotoxicity in mice. J. Immunol. 172: 3034-3041
  4. Hsieh, G. C. et al. (2004) A secreted protein from the human hookworm necator americanus binds selectively to NK cells and induces IFN-gamma production. J. Immunol. 173: 2699-2704

Related products for
CD49b (DX5) MicroBeads, mouse

4 products available