CD44 MicroBeads, human

Name: CD44 MicroBeads, human
Availability: InStock

CD44 MicroBeads, human

CD44 MicroBeads have been specifically designed and optimized for the isolation or depletion of CD44

⁺

cells from single-cell suspensions from solid tumors, including breast, pancreatic, colon, and prostate tissues or cancer cell lines.

Product data and images for CD44 MicroBeads, human

Figures

Figure 1

CD44

⁺

cells were isolated from a mixture of U937 (CD44

⁺

) and 1881 (CD44

^–

) cell lines. CD44

⁺

cells were isolated using the CD44 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD44-PE and analyzed by flow cytometry using the MACSQuant® Analyzer.

Unseparated fraction	CD44 ^– cells
View details CD44 MicroBeads, human Figure 1 CD44 ⁺ cells were isolated from a mixture of U937 (CD44 ⁺ ) and 1881 (CD44 ^– ) cell lines. CD44 ⁺ cells were isolated using the CD44 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD44-PE and analyzed by flow cytometry using the MACSQuant® Analyzer.	View details CD44 MicroBeads, human Figure 1 CD44 ⁺ cells were isolated from a mixture of U937 (CD44 ⁺ ) and 1881 (CD44 ^– ) cell lines. CD44 ⁺ cells were isolated using the CD44 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD44-PE and analyzed by flow cytometry using the MACSQuant® Analyzer.
CD44 ⁺ cells
View details CD44 MicroBeads, human Figure 1 CD44 ⁺ cells were isolated from a mixture of U937 (CD44 ⁺ ) and 1881 (CD44 ^– ) cell lines. CD44 ⁺ cells were isolated using the CD44 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD44-PE and analyzed by flow cytometry using the MACSQuant® Analyzer.

Specifications for CD44 MicroBeads, human

Overview

CD44 MicroBeads have been specifically designed and optimized for the isolation or depletion of CD44

⁺

cells from single-cell suspensions from solid tumors, including breast, pancreatic, colon, and prostate tissues or cancer cell lines.

Detailed product information

Background information

CD44 is a marker for many types of cancer stem cells (CSC), including breast CSCs that possess higher tumorigenicity and metastatic potential

¹

, colorectal

²

, pancreatic

³

, and prostate

^4,5

CSCs. In addition, expression was observed in several cancers as well as on carcinoma cell lines. Here, it plays a role in cancer cell migration and matrix adhesion in response to a cellular microenvironment, thus enhancing cellular aggregation and tumor cell growth

⁶

. CD44 is also expressed on mesodermal cells, such as hematopoietic, fibroblastic, and glial cells.

Columns

For positive selection: LS, XS, or autoMACS

^®

Columns. For depletion: LD, CS, D, or autoMACS Columns.

Note: This product is not suitable for cell separation with an MS Column.

Resources for CD44 MicroBeads, human

Documents and Protocols

Brochures/posters

Cancer Research - Understanding cancer begins with the cell

Scientific posters

Magnetic isolation and rare cell gene expression analysis of breast cancer stem cells

Scientific posters

Isolation of tumor cell subpopulations using semi-automated tissue dissociation and magnetic cell separation

References for CD44 MicroBeads, human

Publications

Al-Hajj, M. et al. (2003) Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U.S.A. 100: 3983-3988
Dalerba, P. et al. (2007) Phenotypic characterization of human colorectal cancer stem cells. Proc. Natl. Acad. Sci. U.S.A. 104: 10158-10163
Li, C. P. et al. (2007) Identification of pancreatic cancer stem cells. Cancer Res. 67: 1030-1037
Patrawala, L. et al. (2006)
Highly purified CD44
⁺
prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells.
Oncogene 25: 1696-1708
Collins, A. T. et al. (2005) Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res. 65: 10946-10951
Aruffo, A. et al. (1990) CD44 is the principal cell surface receptor for hyaluronate. Cell 61: 1303-1313

Data and images