CD39 Antibody, anti-human

Name: CD39 Antibody, anti-human
Availability: InStock

CD39 Antibody, anti-humanExtended Validation

Clone:

MZ18-23C8

Type of antibody:

Primary antibodies

Isotype:

mouse IgG1κ

Applications:

FC, MICS, IF, IHC, ICC

Alternative names:

Entpd1, ATPDase, NTPDase-1, SPG64, EC3.6.1.5, gp80

Flow cytometry applications forCD39 Antibody, anti-human

APC
APC-Vio 770
Biotin
FITC
PE
PE-Vio 770
VioBlue
pure

Conjugate:

APC

Recommended dilution:

1:11

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-100-459, 130-093-504

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:11)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with CD39 antibodies or with the corresponding isotype control antibodies (left images) as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. Furthermore, to evaluate CD39 expression on regulatory T cells, CD4⁺ T cells were enriched using the CD4⁺T Cell Isolation Kit, human. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

MICS (MACSima Imaging Cyclic Staining) applications forCD39 Antibody, anti-human

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:11

Fixation:

PFA

Tested:

during development

Species tested:

human

Products used:

130-100-459, 130-093-504

Compatible applications:

IF, IHC, ICC

Protocols:

Protocol for MICS experiments

Conjugate:

APC

Recommended dilution:

1:11

Fixation:

Acetone

Tested:

during development

Species tested:

human

Products used:

130-100-459, 130-093-504

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Extended validation for CD39 Antibody, anti-human

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with MZ18-23C8
REA739	++
A1	++
TU66	++
Cells were incubated with an excess of purified unconjugated CD39 (MZ18-23C8) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD39. Human peripheral blood mononuclear cells (PBMCs) were stained with CD39 antibodies and with a suitable counterstaining. As a control, CD39 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD39. Human peripheral blood mononuclear cells (PBMCs) were stained with CD39 antibodies and with a suitable counterstaining. As a control, CD39 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD39. Human peripheral blood mononuclear cells (PBMCs) were stained with CD39 antibodies and with a suitable counterstaining. As a control, CD39 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD39. Human peripheral blood mononuclear cells (PBMCs) were stained with CD39 antibodies and with a suitable counterstaining. As a control, CD39 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD39. Human peripheral blood mononuclear cells (PBMCs) were stained with CD39 antibodies and with a suitable counterstaining. As a control, CD39 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD39 (MZ18-23C8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD39 (MZ18-23C8). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD39 Antibody, anti-human

Overview

The CD39 antibody specifically recognizes the membrane-bound human CD39 antigen, which is expressed on an effector/memory-like subset of FoxP3

⁺

regulatory T cells. CD39 is an ectonucleotidase and catalyzes the hydrolysis of extracellular nucleotides, for example, ATP. In concert with CD73, which is an ecto-5’-nucleotidase, this can lead to the production of adenosine. High extracellular ATP concentrations indicate tissue injury and cell death and induce various pro-inflammatory responses in immune cells.

Through its enzymatic activity, CD39 can contribute to the suppressive function of regulatory T cells, for example, by eliminating extracellular ATP or by generating adenosine, which has suppressive effects on various immune cells.

Alternative names

Entpd1, ATPDase, NTPDase-1, SPG64, EC3.6.1.5, gp80

Detailed product information

Technical specifications

Clone	MZ18-23C8
Clonality	monoclonal
Isotype	mouse IgG1κ
Isotype control	Isotype Control Antibody, mouse IgG1
Host	mouse
Type of antibody	Primary antibodies
Species	human, non-human primate
Cross-reactivity	rhesus monkey ( Macaca mulatta )
Antigen	CD39
Alternative names of antigen	Entpd1, ATPDase, NTPDase-1, SPG64, EC3.6.1.5, gp80
Molecular mass of antigen [kDa]	58
Distribution of antigen	B cells, dendritic cells, endothelial cells, Langerhans cells, macrophages, monocytes, NK cells, platelets, T cells, lymphocytes, placenta
Entrez Gene ID	953
RRID	AB_2922171, AB_2922147, AB_2811318, AB_2889572, AB_2889571, AB_2660867, AB_1036210, AB_2660872, AB_2660873, AB_1036212, AB_1036208, AB_2904983, AB_2893087, AB_2811320

Resources for CD39 Antibody, anti-human

Certificates

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Reviews for CD39 Antibody, anti-human

CD39 Antibody of Good Quality from Miltenyi Biotec

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CD39-FITC, human (130-093-502)

Will buy and use it again. Recommendable!

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References for CD39 Antibody, anti-human

Publications

Borsellino, G. et al. (2007)
Expression of ectonucleotidase CD39 by FoxP3
⁺
reg cells: hydrolysis of extracellular ATP and immune suppression.
Blood 110: 1225-1232

Applications

Extended validation