CD370 (CLEC9A) Antibody, anti-human, REAfinity™
Clone:
REA976
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
DNGR-1, C-type lectin family member 9A

Extended validation for CD370 (CLEC9A) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA976
8F9++
3A4/Clec9A+
9A11++
683409 (mIgG1)++
Cells were incubated with an excess of purified unconjugated CD370 (CLEC9A) (REA976) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD370 (CLEC9A) (REA976). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD370 (CLEC9A) (REA976). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD370 (CLEC9A) (REA976). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD370 (CLEC9A) Antibody, anti-human, REAfinity™

Overview

Clone REA976 recognizes the human CD370 antigen, also known as C-type lectin domain family 9 member A (CLEC9A). CD370 is a type II transmembrane glycoprotein member of 241 amino acids with a predicted molecular mass of ~30 kDa. It is also known as DNGR-1 and is expressed on BDCA-3
+
myeloid dendritic cells from peripheral blood and lymphoid tissues. CD370 acts as a receptor for necrotic cells and plays an important role in cross-presentation.
Additional information: Clone REA976 displays negligible binding to Fc receptors.

Alternative names

DNGR-1, C-type lectin family member 9A

Detailed product information

Technical specifications

CloneREA976
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD370 (CLEC9A)
Alternative names of antigenDNGR-1, C-type lectin family member 9A
Molecular mass of antigen [kDa]27
Distribution of antigendendritic cells, myeloid dendritic cells
Entrez Gene ID283420
RRIDAB_2727482, AB_2727523, AB_2727483, AB_2727524, AB_2727484, AB_2727521, AB_2727481, AB_2801887, AB_2922242, AB_2922198, AB_2727522

Resources for CD370 (CLEC9A) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD370 (CLEC9A) Antibody, anti-human, REAfinity™

Publications

  1. Poulin, L. F. et al. (2010)
    Characterization of human DNGR-1
    +
    BDCA3
    +
    leukocytes as putative equivalents of mouse CD8 alpha
    +
    dendritic cells.
    J. Exp. Med. 207(6): 1261-1271
  2. Sancho, D. et al. (2008) Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J. Clin. Invest. 118(6): 2098-2110
  3. Sancho, D. et al. (2009) Identification of a dendritic cell receptor that couples sensing of necrosis to immunity. Nature 458(7240): 899-903

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