The CD34 MicroBead Kit has been developed for the positive selection of CD34
+
hematopoietic stem and progenitor cells in a single step. CD34
+
cells can be isolated from:
  • peripheral blood (PBMCs)
  • bone marrow1
  • leukapheresis product2
  • cord blood3
  • differentiated ES and iPS cell lines4,5.

Data and images for CD34 MicroBead Kit, human

Figures

Figure 1

Flow cytometric analysis of CD34
+
cells isolated from bone marrow mononuclear cells (BM-MNCs) using the CD34 MicroBead Kit, an MS Column, and a MiniMACS™ Separator.
BM-MNCs before separation
Isolated CD34
+
cells
View details

Figure 1

Flow cytometric analysis of CD34
+
cells isolated from bone marrow mononuclear cells (BM-MNCs) using the CD34 MicroBead Kit, an MS Column, and a MiniMACS™ Separator.
View details

Figure 1

Flow cytometric analysis of CD34
+
cells isolated from bone marrow mononuclear cells (BM-MNCs) using the CD34 MicroBead Kit, an MS Column, and a MiniMACS™ Separator.

Specifications for CD34 MicroBead Kit, human

Overview

The CD34 MicroBead Kit has been developed for the positive selection of CD34
+
hematopoietic stem and progenitor cells in a single step. CD34
+
cells can be isolated from:
  • peripheral blood (PBMCs)
  • bone marrow1
  • leukapheresis product2
  • cord blood3
  • differentiated ES and iPS cell lines4,5.

Detailed product information

Background information

CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts
5,6
, endothelial progenitor cells
5,7-9
, and mature endothelial cells
8
.
Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.

Downstream applications

Cells isolated with the CD34 MicroBead Kit are suitable for various downstream applications, including:
  • flow cytometric analysis
  • expansion in culture
  • in vitro differentiation5
  • reprogramming3
  • retroviral transduction10
  • gene expression analyses
  • transplantation into humanized mice

Columns

MS, LS, XS, or autoMACS
®
Columns.

Reviews for CD34 MicroBead Kit, human

CD34 Microbeads Review

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CD34 MicroBead Kit, human (130-046-702)

We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. One of the approaches to do so, is to reprogram T cells at the stem cell level. As such, we use the CD34 Microbeads for stem cells purification from leukopacks.

Beads For Isolation Of Hematopoietic Stem Cells From PBMCs

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CD34 MicroBead Kit, human (130-046-702)

Our lab has developed different mouse models to study the efficacy of therapeutic candidates against HIV. One of the models we have generated consists of injecting human hematopoietic stem cells into immunocompromised NSG mice. We later infect these mice with HIV to study different aspects of infection in vivo. We use this kit to purify human hematopoietic stem cells from blood.

References for CD34 MicroBead Kit, human

Publications

  1. Brault, J. et al. (2014)
    Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
    ex vivo
    models of X
    0
    -linked, AR22
    0
    - and AR47
    0
    - chronic granulomatous diseases.
    Biores Open Access 3(6): 311-326
  2. Schreiber, T. D. et al. (2009) The integrin alpha9beta1 on hematopoietic stem and progenitor cells: involvement in cell adhesion, proliferation and differentiation. Haematologica 94: 1493-1501
  3. Jing et al. (2010) CD49d blockade by natalizumab in patients with multiple sclerosis affects steady-state hematopoiesis and mobilizes progenitors with a distinct phenotype and function. Bone Marrow Transplant. 45: 1489-1496
  4. Ban, H. et al. (2011) Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. Proc. Natl. Acad. Sci. U.S.A. 108: 14234-9
  5. Bandi, S. and Akkina, R. (2008) Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection. AIDS Res. Ther. 5: 1
  6. Wang, Z. Z. et al. (2007) Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo. Nat. Biotechnol. 25: 317-318
  7. Loges, S. et al. (2004) Identification of the adult human hemangioblast. Stem Cells Dev. 13: 229-242
  8. Peichev, M. et al. (2000)
    Expression of VEGFR-2 and AC133 by circulating human CD34
    +
    cells identifies a population of functional endothelial precursors.
    Blood 95: 952-958
  9. Rafii, S. and Lyden, D. (2003) Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat Med 9: 702-712
  10. Valgimigli, M. et al. (2004)
    CD34
    +
    and endothelial progenitor cells in patients with various degrees of congestive heart failure.
    Circulation 110: 1209-1212
  11. Chen et al. (2011) Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in mice. Blood 117: 1530-1539

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