CD33 MicroBeads have been developed for separation of cells based on the expression of the CD33 antigen. CD33
+
cells can be enriched from PBMCs, bone marrow¹
,
², or tissues.³

Data and images for CD33 MicroBeads, human

Figures

Figure 1

Separation of CD33
+
cells from human PBMCs using CD33 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells are fluorescently stained with CD33-PE and CD14-APC.
PBMCs before separation
Isolated CD33
+
cells
View details

Figure 1

Separation of CD33
+
cells from human PBMCs using CD33 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells are fluorescently stained with CD33-PE and CD14-APC.
View details

Figure 1

Separation of CD33
+
cells from human PBMCs using CD33 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells are fluorescently stained with CD33-PE and CD14-APC.

Specifications for CD33 MicroBeads, human

Overview

CD33 MicroBeads have been developed for separation of cells based on the expression of the CD33 antigen. CD33
+
cells can be enriched from PBMCs, bone marrow¹
,
², or tissues.³

Detailed product information

Background information

The CD33 antigen is brightly expressed on monocytes and dimly on granulocytes and dendritic cells. The CD33 antigen is also found on myeloid progenitor cells but is not expressed on lymphocytes, platelets, erythrocytes, and primitive hematopoietic stem cells.

Applications

CD33
+
cells can be enriched from PBMCs, bone marrow
1,2
, or tissues.
3
Additionally, the CD33 antigen can be used to distinguish acute myeloid leukemias from lymphoblastic leukemias.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Resources for CD33 MicroBeads, human

References for CD33 MicroBeads, human

Publications

  1. Czerniecki, B. J. et al. (1997) Calcium ionophore-treated peripheral blood monocytes and dendritic cells rapidly display characteristics of activated dendritic cells. J. Immunol. 159: 3823-3837
  2. Gonzalez, R. et al. (1999) Transduction of bone marrow cells by the AdZ.F(pK7) modified adenovirus demonstrates preferential gene transfer in myeloma cells. Hum. Gene Ther. 10: 2709-2717
  3. Rogler et al. (1998) Isolation and phenotypic characterization of colonic macrophages. Clin. Exp. Immunol. 112: 205-215

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