CD326 (EpCAM) MicroBeads, mouse

CD326 (EpCAM) MicroBeads have been developed for positive selection or depletion of epithelial cells or epithelial tumor cells comprising from single-cell preparation of tissues and organs expressing mouse CD326 (EpCAM) antigen.

Data and images for CD326 (EpCAM) MicroBeads, mouse

Figures

Figure 1

Tumors induced by 4T1 cell line were dissociated by using the Tumor Dissociation Kit, mouse (# 130-096-730) and the gentleMACS Octo Dissociator (# 130-095-937). CD45
+
cells were depleted by using the CD45 MicroBeads, a MidiMACS™ Separator, and a LS Column. CD326 (EpCAM)
+
cells were isolated by using the CD326 (EpCAM) MicroBeads, a MidiMACS Separator, and a LS Column. The cells were fluorescently stained with CD45-PE (# 130-102-596) and CD326 (EpCAM)-APC (# 130-102-234) and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
Isolated CD45
–
CD326 (EpCAM)
+
cells
View details

Figure 1

Tumors induced by 4T1 cell line were dissociated by using the Tumor Dissociation Kit, mouse (# 130-096-730) and the gentleMACS Octo Dissociator (# 130-095-937). CD45
+
cells were depleted by using the CD45 MicroBeads, a MidiMACS™ Separator, and a LS Column. CD326 (EpCAM)
+
cells were isolated by using the CD326 (EpCAM) MicroBeads, a MidiMACS Separator, and a LS Column. The cells were fluorescently stained with CD45-PE (# 130-102-596) and CD326 (EpCAM)-APC (# 130-102-234) and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Tumors induced by 4T1 cell line were dissociated by using the Tumor Dissociation Kit, mouse (# 130-096-730) and the gentleMACS Octo Dissociator (# 130-095-937). CD45
+
cells were depleted by using the CD45 MicroBeads, a MidiMACS™ Separator, and a LS Column. CD326 (EpCAM)
+
cells were isolated by using the CD326 (EpCAM) MicroBeads, a MidiMACS Separator, and a LS Column. The cells were fluorescently stained with CD45-PE (# 130-102-596) and CD326 (EpCAM)-APC (# 130-102-234) and analyzed by flow cytometry using the MACSQuant Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD326 (EpCAM) MicroBeads, mouse

Overview

CD326 (EpCAM) MicroBeads have been developed for positive selection or depletion of epithelial cells or epithelial tumor cells comprising from single-cell preparation of tissues and organs expressing mouse CD326 (EpCAM) antigen.

Detailed product information

Background information

CD326, also known as epithelial cell adhesion molecule (EpCAM), is seen in the majority of carcinomas. It is expressed on cells of epithelial origin, epithelium-derived tumor cells
1
, circulating tumor cells, and cancer stem cells. Moreover , EpCAM is expressed on mouse embryonic stem cells and was described as a marker for the identification of successfully reprogrammed induced pluripotent stem cells (iPS)
2
.

Downstream applications

Tumor cells enriched by CD326 (EpCAM) MicroBeads can be cultured
3
and analyzed by immunocytochemistry
4
, flow cytometry, or molecular biology techniques, for example, RT-PCR
5
.

Columns

MS, LS, XS, or autoMACS
®
Columns.

References for CD326 (EpCAM) MicroBeads, mouse

Publications

  1. Molloy, T. J. et al. (2008) Towards an optimized platform for the detection, enrichment, and semi-quantitation circulating tumor cells. Breast Cancer Res. Treat. 112: 297-307
  2. Moldenhauer, G. et al. (1987) Epithelium specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br. J. Cancer 56: 714-721
  3. Chen, H. F. et al. (2011) Surface marker epithelial cell adhesion molecule and E-cadherin facilitate the Stem Cell Rev. 7(3): 722-735
  4. Leinung, S. et al. (2000) Cytokeratin‑positive cells in bone marrow in comparison with other prognostic factors in colon carcinoma. Langenbecks Arch. Surg. 385: 337-343
  5. Krüger, W. et al. (2000) Immunomagnetic tumor cell selection--implications for the detection of disseminated cancer cells. Transfusion 40: 1489-1493

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