Clone:
REA202
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
BST2, Tetherin, PDCA1, HM1.24

Extended validation for CD317 (BST2) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA202
26F8++
RS38E++
Cells were incubated with an excess of purified unconjugated CD317 (BST2) (REA202) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD317 (BST2) Antibody, anti-human, REAfinity™

Overview

Clone REA202 recognizes the CD317 antigen, which is a lipid raft-associated type II transmembrane glycoprotein. CD317 is composed of a short N-terminal cytosolic domain, a transmembrane domain, and a C-terminal glycosyl-phosphatidylinositol anchor. Expression of CD317 is upregulated in the presence of IFN-α on T and B cells, is found on the apical surface of polarized epithelial cells, and on myeloma cells. CD317 expression is also found in an intracellular pool and the protein cycles between this intracellular pool and the cell surface in a clathrin dependent manner. It has been shown to play a role in regulating the growth and development of B cells, cell adhesion, cell migration, and in cultured cells. CD317 restricts the release of formed viral particles at the surface of infected cells.
Additional information: Clone REA202 displays negligible binding to Fc receptors.

Alternative names

BST2, Tetherin, PDCA1, HM1.24

Detailed product information

Technical specifications

CloneREA202
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
pigtail monkey (
Macaca nemestrina
)
AntigenCD317 (BST2)
Alternative names of antigenBST2, Tetherin, PDCA1, HM1.24
Distribution of antigenB cells, T cells
Entrez Gene ID684
RRIDAB_2876971, AB_2657385, AB_2657386, AB_2657387, AB_2657388, AB_2657389, AB_2657390, AB_2657391, AB_2657392, AB_2657393, AB_2657394, AB_2876970

Resources for CD317 (BST2) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD317 (BST2) Antibody, anti-human, REAfinity™

Publications

  1. Rollason, R. et al. (2009) A CD317/tetherin–RICH2 complex plays a critical role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. J. Cell Biol. 184: 721-736
  2. Habermann, A. et al. (2010) CD317/Tetherin is enriched in the HIV-1 envelope and downregulated from the plasma membrane upon virus infection. J. Virol. 84: 4646-4658

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