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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Our local employees are always happy to answer your questions. Highly trained and experienced teams in your country can provide quick, helpful, and comprehensive support.
Miltenyi Biotec S.r.l.
Via Paolo Nanni Costa, 30
40133 Bologna
Italy
Phone: +39 051 6 460 411
Fax: +39 051 6 460 499
E-Mail: macs@miltenyibiotec.it
Web: www.miltenyibiotec.com
For immediate technical support, use our live chat. Connect with us
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Human peripheral blood mononuclear cells (PBMCs) were stained with CD3 antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD4 antibodies. Flow cytometry was performed using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
3D-immunofluorescence analysis of a human tumor xenograft fixed with 4% PFA and subjected to whole-mount immunostaining with CD3 Antibody, anti-human, Vio R667 (red). The sample was processed and optically cleared using the MACS® Clearing Kit and imaged with an UltraMicroscope II light sheet instrument. 3D-rendered maximum intensity projection of sample zoom-in (upper left, Rhodamine-lectin in grey), zoom-out (upper right), overview (lower left, autofluorescenec in grey) and video (lower right, Rhodamine Lectin in grey). Scale bars: 300 µm (upper left), 1000 µm (upper right, lower left).
Other clones | Overlap in recognition with REA613 |
---|---|
BW264/56 | ++ |
UCHT1 | ++ |
HIT3 | ++ |
SK7 | ++ |
OKT3 | ++ |
SP34-2 | + |
Cells were incubated with an excess of purified unconjugated CD3 (REA613) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | REA613 |
---|---|
Clonality | monoclonal |
Isotype | recombinant human IgG1 |
Isotype control | REA Control Antibody (S), human IgG1 |
Host | human cell line |
Type of antibody | Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | CD3 |
Alternative names of antigen | CD3-epsilon, CD3e, Leu4, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, IMD18, TCRE |
Molecular mass of antigen [kDa] | 21 |
Distribution of antigen | leukocytes, NK cells, T cells, thymocytes, CD4+ T cells, CD8+ T cells, NKT cells, T helper cells |
Entrez Gene ID | 916 |
RRID | AB_2725966, AB_2726242, AB_2725967, AB_2726238, AB_2725963, AB_2726771, AB_2726687, AB_2726245, AB_2725970, AB_2726772, AB_2726688, AB_2726243, AB_2725968, AB_2726239, AB_2725964, AB_2726244, AB_2725969, AB_2726240, AB_2725965, AB_2801863, AB_2889574, AB_2889573, AB_2904991, AB_2904990, AB_2904992, AB_2726241 |
Other clones | Overlap in recognition with REA613 |
---|---|
BW264/56 | ++ |
UCHT1 | ++ |
HIT3 | ++ |
SK7 | ++ |
OKT3 | ++ |
SP34-2 | + |
Cells were incubated with an excess of purified unconjugated CD3 (REA613) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. |
Clone | REA613 |
---|---|
Clonality | monoclonal |
Isotype | recombinant human IgG1 |
Isotype control | REA Control Antibody (S), human IgG1 |
Host | human cell line |
Type of antibody | Primary antibodies, Recombinant antibodies |
Species | human |
Antigen | CD3 |
Alternative names of antigen | CD3-epsilon, CD3e, Leu4, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, IMD18, TCRE |
Molecular mass of antigen [kDa] | 21 |
Distribution of antigen | leukocytes, NK cells, T cells, thymocytes, CD4+ T cells, CD8+ T cells, NKT cells, T helper cells |
Entrez Gene ID | 916 |
RRID | AB_2725966, AB_2726242, AB_2725967, AB_2726238, AB_2725963, AB_2726771, AB_2726687, AB_2726245, AB_2725970, AB_2726772, AB_2726688, AB_2726243, AB_2725968, AB_2726239, AB_2725964, AB_2726244, AB_2725969, AB_2726240, AB_2725965, AB_2801863, AB_2889574, AB_2889573, AB_2904991, AB_2904990, AB_2904992, AB_2726241 |
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