CD29 Antibody, anti-human, REAfinity™
Clone:
REA1060
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ITGB1, FNRB, GPIIa, MDF2, MSK12, VLA-BETA, VLAB

Extended validation for CD29 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1060
TS2/16++
MAR4-
HUTS-21-
4B7R-
Cells were incubated with an excess of purified unconjugated CD29 (REA1060) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD29. Human peripheral blood mononuclear cells (PBMCs) were stained with CD29 antibodies and with a suitable counterstaining. As a control, CD29 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD29. Human peripheral blood mononuclear cells (PBMCs) were stained with CD29 antibodies and with a suitable counterstaining. As a control, CD29 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD29. Human peripheral blood mononuclear cells (PBMCs) were stained with CD29 antibodies and with a suitable counterstaining. As a control, CD29 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD29. Human peripheral blood mononuclear cells (PBMCs) were stained with CD29 antibodies and with a suitable counterstaining. As a control, CD29 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD29. Human peripheral blood mononuclear cells (PBMCs) were stained with CD29 antibodies and with a suitable counterstaining. As a control, CD29 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD29. Human peripheral blood mononuclear cells (PBMCs) were stained with CD29 antibodies and with a suitable counterstaining. As a control, CD29 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD29. Human peripheral blood mononuclear cells (PBMCs) were stained with CD29 antibodies and with a suitable counterstaining. As a control, CD29 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD29. Human peripheral blood mononuclear cells (PBMCs) were stained with CD29 antibodies and with a suitable counterstaining. As a control, CD29 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD29. Human peripheral blood mononuclear cells (PBMCs) were stained with CD29 antibodies and with a suitable counterstaining. As a control, CD29 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD29. Human peripheral blood mononuclear cells (PBMCs) were stained with CD29 antibodies and with a suitable counterstaining. As a control, CD29 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD29 (REA1060). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD29 (REA1060). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD29 (REA1060). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD29 (REA1060). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD29 (REA1060). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD29 Antibody, anti-human, REAfinity™

Overview

Clone REA1060 recognizes the human CD29 antigen, a 130 kDa single-pass type I membrane protein. CD29 is also known as integrin β1 or GPIIa. It forms heterodimers with integrins α1-11 and αV to form functional integrin receptors which bind several cell surface and extracellular molecules such as collagen, fibronectin, laminin, VCAM1, cytotactin, osteopontin, epiligrin, thrombospondin, etc. The cytoplasmic domain of CD29 can interact with cytoskeletal proteins and signaling molecules. In humans, CD29 can be found in four different isoforms (β1A, β1B, β1C, β1D).
Additional information: Clone REA1060 displays negligible binding to Fc receptors.

Alternative names

ITGB1, FNRB, GPIIa, MDF2, MSK12, VLA-BETA, VLAB

Detailed product information

Technical specifications

CloneREA1060
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD29
Alternative names of antigenITGB1, FNRB, GPIIa, MDF2, MSK12, VLA-BETA, VLAB
Molecular mass of antigen [kDa]86
Entrez Gene ID3688
RRIDAB_2751459, AB_2751453, AB_2751460, AB_2751454, AB_2921727, AB_2921723, AB_2921728, AB_2921724, AB_2751461, AB_2751455, AB_2904915, AB_2904914, AB_2921874, AB_2921862

Resources for CD29 Antibody, anti-human, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD29 Antibody, anti-human, REAfinity™

Publications

  1. Altruda, F. et al. (1990) A human integrin beta 1 subunit with a unique cytoplasmic domain generated by alternative mRNA processing. Gene 95: 261-266
  2. Shih, D. T. et al. (1993) Structure/function analysis of the integrin beta 1 subunit by epitope mapping. J. Cell Biol. 122(6): 1361-1371
  3. Brakebusch, C. et al. (1997) Genetic analysis of beta1 integrin function: confirmed, new and revised roles for a crucial family of cell adhesion molecules. J. Cell. Sci. 110: 2895-2904

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